Rabbit MYBPC3 Polyclonal Antibody | anti-MYBPC3 antibody

MYBPC3, NT (MYBPC3, Myosin-binding protein C, cardiac-type, C-protein, cardiac muscle isoform) (FITC)

Cat. Num. AAA6323157

• Gene Names: MYBPC3; FHC; CMH4; MYBP-C
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MYBPC3; Polyclonal Antibody; NT (MYBPC3; Myosin-binding protein C; cardiac-type; C-protein; cardiac muscle isoform) (FITC); cardiac muscle isoform; anti-MYBPC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MYBPC3. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYBPC3 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 189-218 in the N-terminal region of human MYBPC3. Swiss/UniProt Accession: Q14896.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(All lanes: MBS6004049 (1:1000-1:2000) Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 141kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MYBPC3 in paraformaldehyde-fixed, paraffin-embedded sections of human heart tissue sections usingMBS6004049. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS6004049 (1:25) for 1hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MYBPC3 in paraformaldehyde-fixed, paraffin-embedded sections of human heart tissue sections usingMBS6004049. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS6004049 (1:25) for 1hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MYBPC3 in paraformaldehyde-fixed, paraffin-embedded sections of human heart tissue sections usingMBS6004049. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS6004049 (1:25) for 1hr at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Product Categories/Family for anti-MYBPC3 antibody

Antibodies; Binding Proteins

References

1. Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Millat, G., et al. Eur J Med Genet 53(5):261-267(2010). 4. Zimmerman, R.S., et al. Genet. Med. 12(5):268-278(2010). 5. Brion, M., et al. Ann. Clin. Lab. Sci. 40(3):285-289(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 148596957
• NCBI GeneID: 4607
• NCBI Accession #: NP_000247.2
• NCBI GenBank Nucleotide #: NM_000256.3
• UniProt Accession #: Q14896; Q16410; Q6R2F7; Q9UE27; Q9UM53; A5PL00
• Molecular Weight: 140,762 Da
• NCBI Official Full Name: myosin-binding protein C, cardiac-type
• NCBI Official Synonym Full Names: myosin binding protein C, cardiac
• NCBI Official Symbol: MYBPC3
• NCBI Official Synonym Symbols: FHC; CMH4; MYBP-C
• NCBI Protein Information: myosin-binding protein C, cardiac-type; C-protein, cardiac muscle isoform
• UniProt Protein Name: Myosin-binding protein C, cardiac-type
• Protein Family: Myosin-binding protein
• UniProt Gene Name: MYBPC3
• UniProt Synonym Gene Names: Cardiac MyBP-C
• UniProt Entry Name: MYPC3_HUMAN

NCBI Description

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family.

Protein type: Myosin-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band

Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding

Biological Process: heart morphogenesis; regulation of heart rate; positive regulation of ATPase activity; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; cell adhesion; myosin filament assembly; muscle filament sliding; cardiac muscle contraction

Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4