Western Blot (WB)
(Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates.)
Rabbit PRAMEF20/21 Polyclonal Antibody | anti-PRAMEF20/21 antibody
Anti-PRAMEF20/21 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
PRAMEF20/21; Polyclonal Antibody; Anti-PRAMEF20/21 Antibody; PRAME family member 20/21; anti-PRAMEF20/21 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PRAMEF20/21 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-PRAMEF20/21 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant fusion protein of human PRAMEF20/21. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PRAMEF20/21 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PRAMEF20/21 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-PRAMEF20/21 antibody
Rabbit polyclonal antibody to PRAMEF20/21
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,770 Da
NCBI Official Full Name
PRAME family member 20/21
NCBI Official Synonym Full Names
PRAME family member 20
NCBI Official Symbol
PRAMEF20
NCBI Protein Information
PRAME family member 20/21
UniProt Protein Name
PRAME family member 20/21
UniProt Gene Name
PRAMEF20
UniProt Entry Name
PRA20_HUMAN
Uniprot Description
PRAMEF20: Belongs to the PRAME family.
Chromosomal Location of Human Ortholog: 1p36.21
Molecular Function: retinoic acid receptor binding
Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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Product Notes
The PRAMEF20/21 pramef20 (Catalog #AAA8307773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRAMEF20/21 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRAMEF20/21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PRAMEF20/21 pramef20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAMEF20/21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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