Rabbit LHX4 Polyclonal Antibody | anti-LHX4 antibody

Anti-LHX4 Antibody

Cat. Num. AAA8307149

• Gene Names: LHX4; CPHD4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: LHX4; Polyclonal Antibody; Anti-LHX4 Antibody; LIM/homeobox protein Lhx4; LIM homeobox protein 4; anti-LHX4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of LHX4 protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-LHX4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human LHX4. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of LHX4 expression in mouse brain (A), rat brain (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of LHX4 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-LHX4 antibody

Rabbit polyclonal antibody to LHX4

NCBI and Uniprot Product Information

• NCBI GI #: 15375314
• NCBI GeneID: 89884
• NCBI Accession #: NP_203129.1
• NCBI GenBank Nucleotide #: NM_033343.3
• UniProt Accession #: Q969G2; P53776
• Molecular Weight: 43,124 Da
• NCBI Official Full Name: LIM/homeobox protein Lhx4
• NCBI Official Synonym Full Names: LIM homeobox 4
• NCBI Official Symbol: LHX4
• NCBI Official Synonym Symbols: CPHD4
• NCBI Protein Information: LIM/homeobox protein Lhx4; LIM homeobox protein 4
• UniProt Protein Name: LIM/homeobox protein Lhx4
• Protein Family: LIM/homeobox protein
• UniProt Gene Name: LHX4
• UniProt Synonym Gene Names: LIM homeobox protein 4
• UniProt Entry Name: LHX4_HUMAN

NCBI Description

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor involved in the control of differentiation and development of the pituitary gland. Mutations in this gene cause combined pituitary hormone deficiency 4. [provided by RefSeq, Dec 2010]

Uniprot Description

LHX4: May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung. Defects in LHX4 are the cause of pituitary hormone deficiency combined type 4 (CPHD4); also known as short stature pituitary and cerebellar defects and small sella turcica. The disorder is characterized by short stature, pituitary and cerebellar defects, and small transverse depression crossing the midline on the superior surface of the body of the sphenoid bone which houses the pituitary gland. A chromosomal aberration involving LHX4 may be a cause of acute lymphoblastic leukemia. Translocation t(1;14)(q25;q32) with IGHG1.

Protein type: Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: organ morphogenesis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; medial motor column neuron differentiation; motor axon guidance; negative regulation of apoptosis; placenta development

Disease: Pituitary Hormone Deficiency, Combined, 4