Western Blot (WB)
(Anti-PPP1R15B antibody, MBS175717, Western blottingAll lanes: Anti PPP1R15B(MBS175717) at 0.5ug/mlWB: A549 Whole Cell Lysate at 40ugPredicted bind size: 79KDObserved bind size: 79KD )
Rabbit anti-Human PPP1R15B Polyclonal Antibody | anti-PPP1R15B antibody
Anti-PPP1R15B antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
PPP1R15B; Polyclonal Antibody; Anti-PPP1R15B antibody; Protein phosphatase 1 regulatory subunit 15B; protein phosphatase 1; regulatory subunit 15B; CREP antibody; FLJ14744 antibody; PPP1R15B antibody; Protein phosphatase 1; regulatory(inhibitor) subunit 15B antibody; regulatory subunit 15B antibody; anti-PPP1R15B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
713
Applicable Applications for anti-PPP1R15B antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R15B (15-31aa RAGFRFWPPFFPRRSQA).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-PPP1R15B antibody, MBS175717, Western blottingAll lanes: Anti PPP1R15B(MBS175717) at 0.5ug/mlWB: A549 Whole Cell Lysate at 40ugPredicted bind size: 79KDObserved bind size: 79KD )
Western Blot (WB)
(Anti-PPP1R15B antibody, MBS175717, Western blottingAll lanes: Anti PPP1R15B(MBS175717) at 0.5ug/mlWB: A549 Whole Cell Lysate at 40ugPredicted bind size: 79KDObserved bind size: 79KD )
Related Product Information for anti-PPP1R15B antibody
Description: Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) detection. Tested with WB in Human.
Background: PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.
Background: PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
79,152 Da
NCBI Official Full Name
Protein phosphatase 1 regulatory subunit 15B
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 15B
Protein Family
UniProt Gene Name
PPP1R15B
UniProt Entry Name
PR15B_HUMAN
Uniprot Description
PPP1R15B: Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1. Belongs to the PPP1R15 family.
Protein type: Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: protein phosphatase type 1 complex
Molecular Function: protein binding; protein serine/threonine phosphatase activity
Biological Process: regulation of translation; response to hydrogen peroxide; ER overload response
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Product Notes
The PPP1R15B ppp1r15b (Catalog #AAA175717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPP1R15B antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R15B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PPP1R15B ppp1r15b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1R15B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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