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Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP4C antibody.)
Rabbit PPP4C Polyclonal Antibody | anti-PPP4C antibody
PPP4C Antibody
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
PPP4C; Polyclonal Antibody; PPP4C Antibody; PP4; PPX; PP4C; PPH3; PPP4; anti-PPP4C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total PPP4C protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
307
Applicable Applications for anti-PPP4C antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human PPP4C.
Target Name
PPP4C
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP4C antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP4C antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat lung using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat lung using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat spleen using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat spleen using PPP4C antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-PPP4C antibody
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
Product Categories/Family for anti-PPP4C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,080 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 4, catalytic subunit
NCBI Official Symbol
PPP4C
NCBI Official Synonym Symbols
PP4; PPX; PP-X; PP4C; PPH3; PPP4
NCBI Protein Information
serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Gene Name
PPP4C
UniProt Synonym Gene Names
PPP4; PPX; PP4C; Pp4; PP-X
UniProt Entry Name
PP4C_HUMAN
Uniprot Description
PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.
Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus
Molecular Function: NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity
Biological Process: dephosphorylation
Research Articles on PPP4C
Similar Products
Product Notes
The PPP4C ppp4c (Catalog #AAA9407739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP4C Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP4C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the PPP4C ppp4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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