PPP4C recombinant protein

PPP4C, 1-307aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205027

• Gene Names: PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PPP4C; 1-307aa; Human; His tag; E Coli (Denatured); Serine/threonine-protein phosphatase 4 catalytic subunit; PP4; PP4C; PPH3; PPP4; PPX; PPP4C recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL
• Sequence Length: 307

• Applicable Applications for PPP4C recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPP4C recombinant protein

Serine/threonine-protein phosphatase 4 catalytic subunit, also known as PPP4C, belongs to the Serine/threonine-protein phosphatase catalytic subunits. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPP4C is a putative member of a novel PP family. The PPP4C may play a role in dephosphorylation and regulation of HDAC3. Recombinant human PPP4C protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for PPP4C recombinant protein

Signal Transduction

References

Cohen P T., et al. (1993) Biochem Soc Trans. 21:884-888.; Mumby M C., et al. (1993) Phys Rev. 73:673-699.

NCBI and Uniprot Product Information

• NCBI GI #: 4506027
• NCBI GeneID: 5531
• NCBI Accession #: NP_002711
• NCBI GenBank Nucleotide #: NM_002720.2
• UniProt Accession #: P60510; P33172
• Molecular Weight: 37.5 kDa (330aa)
• NCBI Official Full Name: serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 4 catalytic subunit
• NCBI Official Symbol: PPP4C
• NCBI Official Synonym Symbols: PP4; PPX; PP-X; PP4C; PPH3; PPP4
• NCBI Protein Information: serine/threonine-protein phosphatase 4 catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase 4 catalytic subunit
• UniProt Gene Name: PPP4C
• UniProt Synonym Gene Names: PPP4; PPX; PP4C; Pp4; PP-X

Uniprot Description

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.