Rabbit anti-Human PPP4C Polyclonal Antibody | anti-PPP4C antibody

PPP4C, ID (PPP4C, PPP4, PPX, Serine/threonine-protein phosphatase 4 catalytic subunit, Protein phosphatase X) (PE)

Cat. Num. AAA6336730

• Gene Names: PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP4C; Polyclonal Antibody; ID (PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; Protein phosphatase X) (PE); Protein phosphatase X; anti-PPP4C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PPP4C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the Central region of human PPP4C.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPP4C Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP4C antibody detected the PPP4C protein (arrow).)

Immunohistochemistry (IHC)

(PPP4C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP4C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PPP4C antibody

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).

Product Categories/Family for anti-PPP4C antibody

Antibodies; Enzymes; Phosphatase

References

Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010). Mourtada-Maarabouni, M., et al. Leuk. Res. 33(11):1539-1551(2009). Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009): Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008). Nakada, S., et al. EMBO Rep. 9(10):1019-1026(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506027
• NCBI GeneID: 5531
• NCBI Accession #: NP_002711.1
• NCBI GenBank Nucleotide #: NM_002720.2
• UniProt Accession #: P60510; P33172
• Molecular Weight: 35,080 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 4 catalytic subunit isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 4, catalytic subunit
• NCBI Official Symbol: PPP4C
• NCBI Official Synonym Symbols: PP4; PPX; PP-X; PP4C; PPH3; PPP4
• NCBI Protein Information: serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit; protein phosphatase X, catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase 4 catalytic subunit
• UniProt Gene Name: PPP4C
• UniProt Synonym Gene Names: PPP4; PPX; PP4C; Pp4; PP-X
• UniProt Entry Name: PP4C_HUMAN

Uniprot Description

PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: dephosphorylation