Rabbit anti-Human PPP4C Polyclonal Antibody | anti-PPP4C antibody

PPP4C, ID (PPP4C, PPP4, PPX, Serine/threonine-protein phosphatase 4 catalytic subunit, Protein phosphatase X)

Cat. Num. AAA647213

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PPP4C; Polyclonal Antibody; ID (PPP4C; PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; Protein phosphatase X); Anti -PPP4C; anti-PPP4C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PPP4C antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the Central region of human PPP4C.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP4C Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP4C antibody detected the PPP4C protein (arrow).)

Immunohistochemistry (IHC)

(PPP4C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP4C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PPP4C antibody

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).

Product Categories/Family for anti-PPP4C antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 148877418
• NCBI GeneID: 540398
• UniProt Accession #: A6H772
• Molecular Weight: 35,080 Da
• NCBI Official Full Name: PPP4C protein
• NCBI Official Synonym Full Names: protein phosphatase 4, catalytic subunit
• NCBI Official Symbol: PPP4C
• NCBI Protein Information: serine/threonine-protein phosphatase 4 catalytic subunit; pp4; PP4C; protein phosphatase 4 (formerly X), catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase 4 catalytic subunit
• UniProt Gene Name: PPP4C
• UniProt Synonym Gene Names: PP4C; Pp4
• UniProt Entry Name: PP4C_BOVIN

Uniprot Description

Function: Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration

By similarity. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3

By similarity. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair

By similarity. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase

By similarity. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin

By similarity.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 1 manganese ion per subunit

By similarity.

Subunit structure: Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits

By similarity. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4

By similarity. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2

By similarity. Interacts with REL, NFKB1/p50 and RELA

By similarity. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated)

By similarity. Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2

By similarity. Interacts with HDAC3

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

By similarity.

Sequence similarities: Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.