Thiosulfate Sulfurtransferase Recombinant Protein | TST recombinant protein

Recombinant Thiosulfate Sulfurtransferase (TST)

Cat. Num. AAA2033090

• Gene Names: TST; RDS
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thiosulfate Sulfurtransferase; Recombinant Thiosulfate Sulfurtransferase (TST); RDS; Rhodanese; Thiosulfate Cyanide Transsulfurase; Thiosulfate Thiotransferase; TST recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: VHQVLYRAL VSTKWLAESI RSGRLGPSLR VLDASWYSPG TRQARKEYQE RHVPGASFFD IEECRDTTSP YEMMLPSEAH FGDYVGNLGI SNDTHVVVYD GDDLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLNLS LLKTYEQVLE NLQSKRFQLV DSRAQGRYLG TQPEPDIVGL DSGHIRGSVN MPFMDFLTKD GFEKSPEELR AIFQDKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPETRVS QGKSGKA
• Sequence Length: 297

• Applicable Applications for TST recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 37.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Val2~Ala297
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 395394071
• NCBI GeneID: 7263
• NCBI Accession #: NP_001257412.1
• NCBI GenBank Nucleotide #: NM_001270483.1
• Molecular Weight: 33,429 Da
• NCBI Official Full Name: thiosulfate sulfurtransferase
• NCBI Official Synonym Full Names: thiosulfate sulfurtransferase
• NCBI Official Symbol: TST
• NCBI Official Synonym Symbols: RDS
• NCBI Protein Information: thiosulfate sulfurtransferase
• UniProt Protein Name: Thiosulfate sulfurtransferase
• Protein Family: Toxic shock syndrome toxin
• UniProt Gene Name: TST
• UniProt Entry Name: THTR_HUMAN

NCBI Description

This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

Protein type: Mitochondrial; EC 2.8.1.1; Transferase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: 5S rRNA binding; thiosulfate sulfurtransferase activity

Biological Process: cyanate catabolic process; epithelial cell differentiation; rRNA transport; sulfur amino acid catabolic process; sulfur amino acid metabolic process