Western Blot (WB)
(Staining (2ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat PPP2R5D Polyclonal Antibody | anti-PPP2R5D antibody
Goat anti-PPP2R5D Antibody
Western Blot: Approx 70kDa and 60kDa bands seen in 293 lysate (Predicted MW of 68kDa and 62kDa according to NP_006236 and AAH01175, respectively). Recommended for use at 1-3ug/ml.
Western Blot (WB)
(Staining (2ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (2ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP2R5D: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. By retinoic acid; in neuroblastoma cell lines. Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. Belongs to the phosphatase 2A regulatory subunit B56 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cytosol; nucleoplasm; nucleus; protein phosphatase type 2A complex
Molecular Function: protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity
Biological Process: nervous system development; protein amino acid dephosphorylation; regulation of protein phosphatase type 2A activity; signal transduction
Disease: Mental Retardation, Autosomal Dominant 35
Research Articles on PPP2R5D
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Product Notes
The PPP2R5D ppp2r5d (Catalog #AAA420808) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PPP2R5D Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R5D can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 20000. Western Blot: Approx 70kDa and 60kDa bands seen in 293 lysate (Predicted MW of 68kDa and 62kDa according to NP_006236 and AAH01175, respectively). Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the PPP2R5D ppp2r5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRAEEFLTAS QEAL. It is sometimes possible for the material contained within the vial of "PPP2R5D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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