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Western Blot (WB) (Western Blot (WB) analysis of SEPT2 pAb at 1:1000 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Rabbit Septin 2 Polyclonal Antibody | anti-L136 antibody

Septin 2 (L136) Polyclonal Antibody

Gene Names
SEPTIN2; DIFF6; NEDD5; SEPT2; NEDD-5; Pnutl3; hNedd5
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Septin 2; Polyclonal Antibody; Septin 2 (L136) Polyclonal Antibody; DIFF6; hNEDD5; KIAA0158; Neural precursor cell expressed developmentally down regulated protein 5; Pnutl3; Protein NEDD5; SEPT2; anti-L136 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Septin 2 (L136) polyclonal antibody detects endogenous levels of Septin 2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
361
Applicable Applications for anti-L136 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 108-157 of Human Septin 2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SEPT2 pAb at 1:1000 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of SEPT2 pAb at 1:1000 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))
Related Product Information for anti-L136 antibody
The transcriptional regulation of all septins is complex, resulting in alternatively spliced variants. At least three septins (Septin 1, 2 and 4) are associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Septin 3 (G-Septin), a GTPbinding protein, is highly expressed in brain and is regulated by protein kinase G in neurons. The human SEPT4 (H5/PNUTL2/CDCrREL-2) gene encodes ARTS (for apoptosis-related protein in the TGF beta signaling pathway), which is expressed in many cells and acts to enhance cell death induced by TGF beta or, to a lesser extent, by other apoptotic agents. ARTS is localized to mitochondria and translocates to the nucleus when apoptosis occurs.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 45kDa
NCBI Official Full Name
septin-2 isoform a
NCBI Official Synonym Full Names
septin 2
NCBI Official Symbol
SEPTIN2
NCBI Official Synonym Symbols
DIFF6; NEDD5; SEPT2; NEDD-5; Pnutl3; hNedd5
NCBI Protein Information
septin-2
UniProt Protein Name
Septin-2
UniProt Gene Name
SEPT2
UniProt Synonym Gene Names
DIFF6; KIAA0158; NEDD5; NEDD-5
UniProt Entry Name
SEPT2_HUMAN

Uniprot Description

SEPT2: a member of the septin family of proteins. May be involved in the process of cytokinesis. Accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody. In interphase and postmitotic cells, localized to fibrous or granular structures, depending on the growth state of the cell.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Cell development/differentiation; Cell cycle regulation; Hydrolase

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleolus; exocyst; synapse; spindle; midbody; cell cortex; nucleus; actin cytoskeleton; cleavage furrow

Molecular Function: protein binding; GTP binding; protein complex scaffold; enzyme regulator activity

Biological Process: mitosis; smoothened signaling pathway; regulation of protein localization; cell division; regulation of catalytic activity; cilium biogenesis; regulation of L-glutamate transport; neurite development

Research Articles on L136

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Product Notes

The L136 sept2 (Catalog #AAA3004465) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Septin 2 (L136) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Septin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the L136 sept2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Septin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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