Rabbit PPP1CC Polyclonal Antibody | anti-PPP1CC antibody

Anti-PPP1CC Antibody, Rabbit Polyclonal

Cat. Num. AAA8103688

• Gene Names: PPP1CC; PP1C; PP-1G; PPP1G
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, Xenopus tropicalis (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: PPP1CC; Polyclonal Antibody; Anti-PPP1CC Antibody; Rabbit Polyclonal; PPP1CC Antibody; Rabbit PAb; Antigen Affinity Purified; protein phosphatase 1; catalytic subunit; gamma isozyme; Anti-PP-1G Antibody; Anti-PP1C Antibody; Anti-PPP1G Antibody; anti-PPP1CC antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, Xenopus tropicalis (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PPP1CC
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PPP1CC antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PPP1CC
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PPP1CC, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PPP1CC rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:37 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of PPP1CC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PPP1CC polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)

Related Product Information for anti-PPP1CC antibody

Protein phosphatase 1, catalytic subunit, gamma isozyme (PPP1CC) participates in cell proliferation, differentiation, and apoptosis. Using fluorescent in situ hybridization (FISH) method, a gene encoding the catalytic subunit of protein phosphatase type 1 gamma (PPP1CC) was mapped to human 12q24.1-q24.2, rat 7 q22, and mouse 10C. These results indicate that the PPP1CC is a member of the conserved synteny group between rat chromosome 7, mouse chromosome 10, and human chromosome 12. The Ppp1cc gene encodes two alternatively spliced variants, PP1 gamma1 (PPP1CC1) and PP1 gamma2 (PPP1CC2). Global deletion of the Ppp1cc gene, which causes loss of both isoforms, results in male infertility due to impaired spermatogenesis. This phenotype was assumed to be due to the loss of PPP1CC2, which is abundant in testis. While PPP1CC2 is predominant, other PP1 isoforms are also expressed in testis.

References

Sinha N, et al. (2013) Selective ablation of ppp1cc gene in testicular germ cells causes oligo-teratozoospermia and infertility in mice. Biol Reprod 89 (5): 128.Saadat M, et al. (1996) Assignment of the gene encoding type 1 gamma protein phosphatase catalytic subunit (ppp1cc) on human, rat, and mouse chromosomes. Jpn J Hum Genet 41 (1): 159-165.MacLeod G, et al. (2014) Ppp1cc2 can form a kinase/phosphatase complex with the testis-specific proteins tssk1 and tsks in the mouse testis. Reproduction 147 (1): 1-12.Liu X, et al. (2017) Ppp1cc is associated with astrocyte and microglia proliferation after traumatic spinal cord injury in rats. Pathol Res Pract 213 (11): 1355-1364.

NCBI and Uniprot Product Information

• NCBI GI #: 548573
• NCBI GeneID: 5501
• UniProt Accession #: P36873
• Molecular Weight: 323
• NCBI Official Full Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• NCBI Official Synonym Full Names: protein phosphatase 1, catalytic subunit, gamma isozyme
• NCBI Official Symbol: PPP1CC
• NCBI Official Synonym Symbols: PP1C; PP-1G; PPP1G
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-gamma catalytic subunit; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CC
• UniProt Synonym Gene Names: PP-1G
• UniProt Entry Name: PP1G_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PPP1CC: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus

Chromosomal Location of Human Ortholog: 12q24.1-q24.2

Cellular Component: focal adhesion; protein complex; mitochondrion; cytoplasm; nucleolus; nuclear speck; midbody; cytosol; nucleus; cleavage furrow

Molecular Function: protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding

Biological Process: glycogen metabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; mitotic cell cycle; circadian regulation of gene expression; regulation of circadian rhythm; protein amino acid dephosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; entrainment of circadian clock by photoperiod