Rabbit anti-Human PPM1D Polyclonal Antibody | anti-PPM1D antibody

PPM1D, CT (Protein Phosphatase 1D, Protein Phosphatase 2C delta Isoform, PP2C-delta, p53-induced Protein Phosphatase 1, Protein Phosphatase Magnesium-dependent 1 delta, WIP1) (Azide free) (HRP)

Cat. Num. AAA6495437

• Gene Names: PPM1D; WIP1; PP2C-DELTA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PPM1D; Polyclonal Antibody; CT (Protein Phosphatase 1D; Protein Phosphatase 2C delta Isoform; PP2C-delta; p53-induced Protein Phosphatase 1; Protein Phosphatase Magnesium-dependent 1 delta; WIP1) (Azide free) (HRP); anti-PPM1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPM1D.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPM1D antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PPM1D (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PPM1D Pab is used in Western blot to detect PPM1D in 293 cell lysate.)

Product Categories/Family for anti-PPM1D antibody

Antibodies; Enzymes; Phosphatase

References

1. Xinna Zhang, et al. Cancer Res. published 6 October 2009, 10.1158/0008-5472. CAN-09-0634. (E-pub). 2. Hye-Sook Han, et al. Mol. Cancer Res. 2009; 7:713-723. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 4505997
• NCBI GeneID: 8493
• NCBI Accession #: NP_003611
• NCBI GenBank Nucleotide #: NM_003620.3
• UniProt Accession #: O15297; Q53XP4; Q6P991; Q8IVR6
• Molecular Weight: Predicted: 67 kDa; ; Observed: 68 kDa
• NCBI Official Full Name: protein phosphatase 1D
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1D
• NCBI Official Symbol: PPM1D
• NCBI Official Synonym Symbols: WIP1; PP2C-DELTA
• NCBI Protein Information: protein phosphatase 1D; protein phosphatase Wip1; wild-type p53-induced phosphatase 1; protein phosphatase 2C delta isoform; protein phosphatase 1D magnesium-dependent, delta isoform
• UniProt Protein Name: Protein phosphatase 1D
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1D
• UniProt Synonym Gene Names: WIP1; PP2C-delta
• UniProt Entry Name: PPM1D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]