Rabbit GABAB R1 Polyclonal Antibody | anti-GABAB antibody

GABAB R1 (I902) Polyclonal Antibody

Cat. Num. AAA3000533

• Gene Names: GABBR1; GB1; GPRC3A; GABABR1; GABBR1-3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GABAB R1; Polyclonal Antibody; GABAB R1 (I902) Polyclonal Antibody; Gamma-aminobutyric acid type B receptor subunit 1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1; GABBR1; GPRC3A; GABB-R1; anti-GABAB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GABAB R1 (I902) polyclonal antibody detects endogenous levels of GABA-B Receptor protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 961

• Applicable Applications for anti-GABAB antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 870-913 of Human GABAB R1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GABAB R1 (I902) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-GABAB antibody

The GABAB R receptor is a heterodimer that consists of two multi-pass membrane proteins, designated GABAB R1 and GABAB R2, both of which belong to the G protein-coupled receptor family and are highly expressed in brain tissue. Together, GABAB R1 and GABAB R2 play a crucial role in the fine-tuning of inhibitory synaptic transmissions and are implicated in slow wave sleep, muscle relaxation, hippocampal long-term potentiation and antinociception events. Both GABAB R1 and GABAB R2 are regulated by G proteins that have a variety of functions, including activation of potassium channels, inhibition of adenylyl cyclase (A cyclase) activity and modulation of inositol phospholipid hydrolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 10835015
• NCBI GeneID: 2550
• NCBI Accession #: NP_001461.1
• NCBI GenBank Nucleotide #: NP_001461.1
• UniProt Accession #: Q9UBS5
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: gamma-aminobutyric acid type B receptor subunit 1 isoform a
• NCBI Official Synonym Full Names: gamma-aminobutyric acid type B receptor subunit 1
• NCBI Official Symbol: GABBR1
• NCBI Official Synonym Symbols: GB1; GPRC3A; GABABR1; GABBR1-3
• NCBI Protein Information: gamma-aminobutyric acid type B receptor subunit 1
• UniProt Protein Name: Gamma-aminobutyric acid type B receptor subunit 1
• UniProt Gene Name: GABBR1
• UniProt Synonym Gene Names: GPRC3A; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1
• UniProt Entry Name: GABR1_HUMAN

NCBI Description

This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA(B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jan 2016]

Uniprot Description

GABBR1: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high- voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen. Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Isoform 1E (without C-terminal intracellular domain) is unable to dimerize via a coiled-coil interaction with GABA-B-R2. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region. Interacts with JAKMIP1. Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 3

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: presynaptic membrane; postsynaptic membrane; integral to plasma membrane; cytoplasm; dendrite; plasma membrane; extracellular region; cell junction

Molecular Function: protein binding; GABA-B receptor activity

Biological Process: synaptic transmission; negative regulation of adenylate cyclase activity; G-protein signaling, adenylate cyclase inhibiting pathway; gamma-aminobutyric acid signaling pathway