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Western Blot (WB) (PPIF Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPIF antibody detected the PPIF protein (arrow).)

Rabbit anti-Human PPIF Polyclonal Antibody | anti-PPIF antibody

PPIF (Peptidylprolyl Isomerase F) (AP)

Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PPIF; Polyclonal Antibody; PPIF (Peptidylprolyl Isomerase F) (AP); Peptidyl-prolyl cis-trans isomerase F Mitochondrial; PPIase F; Cyclophilin D; CyP-D; CypD; CYP3; Cyclophilin F; Rotamase F; anti-PPIF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PPIF antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PPIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the Central region of human PPIF.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPIF Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPIF antibody detected the PPIF protein (arrow).)

Western Blot (WB) (PPIF Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPIF antibody detected the PPIF protein (arrow).)
Related Product Information for anti-PPIF antibody
PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Product Categories/Family for anti-PPIF antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): He, Y., et al. Invest. Ophthalmol. Vis. Sci. 49(11):4912-4922(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Lin, D.T., et al. J. Biol. Chem. 277(34):31134-31141(2002). Bowles, K.R., et al. Genomics 67(2):109-127(2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,040 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
PPIF
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin 3; cyclophilin D; cyclophilin F; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Gene Name
PPIF
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M
UniProt Entry Name
PPIF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.

Protein type: EC 5.2.1.8; Mitochondrial; Isomerase

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding

Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis

Research Articles on PPIF

Similar Products

Product Notes

The PPIF ppif (Catalog #AAA6336280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIF (Peptidylprolyl Isomerase F) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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