Rabbit anti-Human RALA Polyclonal Antibody | anti-RALA antibody

RALA, CT (RALA, RAL, Ras-related protein Ral-A) (AP)

Cat. Num. AAA6340119

• Gene Names: RALA; RAL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RALA; Polyclonal Antibody; CT (RALA; RAL; Ras-related protein Ral-A) (AP); Ras-related protein Ral-A; anti-RALA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RALA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RALA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human RALA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RALA Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RALA antibody detected the RALA protein (arrow).)

Related Product Information for anti-RALA antibody

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

Product Categories/Family for anti-RALA antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Nichols, C.D., et al. Curr. Biol. 20(14):1316-1320(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Godin, C.M., et al. Mol. Pharmacol. 77(3):388-395(2010). Lim, K.H., et al. Mol. Cell. Biol. 30(2):508-523(2010). Wang, K., et al. Int J Immunopathol Pharmacol 22(3):735-743(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 33946329
• NCBI GeneID: 5898
• NCBI Accession #: NP_005393.2
• NCBI GenBank Nucleotide #: NM_005402.3
• UniProt Accession #: P11233; A4D1W3
• Molecular Weight: 23,567 Da
• NCBI Official Full Name: ras-related protein Ral-A
• NCBI Official Synonym Full Names: v-ral simian leukemia viral oncogene homolog A (ras related)
• NCBI Official Symbol: RALA
• NCBI Official Synonym Symbols: RAL
• NCBI Protein Information: ras-related protein Ral-A; RAS-like protein A; Ras family small GTP binding protein RALA; ras related GTP binding protein A
• UniProt Protein Name: Ras-related protein Ral-A
• Protein Family: Ras-related protein
• UniProt Gene Name: RALA
• UniProt Synonym Gene Names: RAL
• UniProt Entry Name: RALA_HUMAN

NCBI Description

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: cytoplasmic vesicle membrane; cell surface; focal adhesion; endocytic vesicle; plasma membrane; midbody; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin binding; Edg-2 lysophosphatidic acid receptor binding; ubiquitin protein ligase binding; ATPase binding

Biological Process: positive regulation of filopodium formation; regulation of exocytosis; exocytosis; lipid raft localization; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; Ras protein signal transduction; actin cytoskeleton reorganization; cytokinesis; chemotaxis; signal transduction