Western Blot (WB)
(Western Blot analysis of Human placenta tissue using PLOD3 Monoclonal Antibody at dilution of 1:300)
Mouse anti-Human PLOD3 Monoclonal Antibody | anti-PLOD3 antibody
PLOD3 Monoclonal Antibody
Gene Names
PLOD3; LH3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Caprylic acid/ammonium sulfate precipitation
Synonyms
PLOD3; Monoclonal Antibody; PLOD3 Monoclonal Antibody; 2-oxoglutarate 5-dioxygenase 3; bone protein I; BP-I; LH3; Lysine hydroxylase 3; Lysyl hydroxylase 3; Plod3; Procollagen lysine; 2 oxoglutarate 5 dioxygenase 3 precursor; Procollagen-lysine; anti-PLOD3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
367
Purity/Purification
Caprylic acid/ammonium sulfate precipitation
Concentration
1mg/mL (varies by lot)
Sequence Length
738
Applicable Applications for anti-PLOD3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
IHC: 1:20-1:200
IF: 1:10-1:100
Immunogen
Fusion protein of PLOD3
Buffer
PBS with 0.1% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of Human placenta tissue using PLOD3 Monoclonal Antibody at dilution of 1:300)
Western Blot (WB)
(Western Blot analysis of Human placenta tissue using PLOD3 Monoclonal Antibody at dilution of 1:300)
Related Product Information for anti-PLOD3 antibody
PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 85kDa
Observed: 80-85kDa
Observed: 80-85kDa
NCBI Official Full Name
procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
NCBI Official Synonym Full Names
procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
NCBI Official Symbol
PLOD3
NCBI Official Synonym Symbols
LH3
NCBI Protein Information
procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
UniProt Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
Protein Family
UniProt Gene Name
PLOD3
UniProt Synonym Gene Names
LH3
NCBI Description
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
Research Articles on PLOD3
Similar Products
Product Notes
The PLOD3 plod3 (Catalog #AAA2542031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PLOD3 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLOD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:200-1:1000 IHC: 1:20-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the PLOD3 plod3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLOD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
