Rabbit PP2A Polyclonal Antibody | anti-PP2A antibody

PP2A (Phospho-Y307) Polyclonal Antibody

Cat. Num. AAA3013986

• Gene Names: PPP2CB; PP2CB; PP2Abeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: PP2A; Polyclonal Antibody; PP2A (Phospho-Y307) Polyclonal Antibody; PP2A-alpha; RP-C; PP2A-beta; PP2A A; PP2A alpha; PP2A B; PP2A beta; PP2AA; PP2Ac; PP2CB; PPP2CA; PPP2CB; Protein phosphatase 2 catalytic subunit alpha isoform; Protein phosphatase 2 catalytic subunit beta isoform; Replication protein C; RP C; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; anti-PP2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PP2A (Phospho-Y307) polyclonal antibody detects endogenous levels of PP2A protein only when phosphorylated at Y307.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 309

• Applicable Applications for anti-PP2A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Phospho-PP2A (pY307) on different lysates using anti-Phospho-PP2A (pY307) antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: F9 Lane 3: PC12)

Immunocytochemistry (ICC)

(ICC staining Phospho-PP2A (pY307) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-PP2A antibody

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development. The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by alpha and beta isoforms. Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain alpha, beta, gamma and delta isoforms, with the B' family also including an epsilon protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit. This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity. Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed. Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits.

NCBI and Uniprot Product Information

• NCBI GI #: 50402236
• NCBI GeneID: 5516
• UniProt Accession #: P62714; P67775; P62715; P63330; P62716 P63331
• Molecular Weight: 35kDa
• NCBI Official Full Name: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 catalytic subunit beta
• NCBI Official Symbol: PPP2CB
• NCBI Official Synonym Symbols: PP2CB; PP2Abeta
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
• Protein Family: PP2A regulatory
• UniProt Gene Name: PPP2CB
• UniProt Synonym Gene Names: PP2A-beta
• UniProt Entry Name: PP2AB_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

PPP2CB: one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: spindle pole; protein phosphatase type 2A complex; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: response to antibiotic; proteasomal ubiquitin-dependent protein catabolic process; apoptotic mitochondrial changes; fibroblast growth factor receptor signaling pathway; response to hydrogen peroxide; regulation of gene expression; negative regulation of Ras protein signal transduction; protein amino acid dephosphorylation