Chromatin Immunoprecipitation (ChIP)
(PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 ul of PP2A antibody or the equivalent amount of IgG as a negative control.)
Rabbit PP2A Polyclonal Antibody | anti-PP2A antibody
PP2A antibody (pAb)
Reactivity
Human, Mouse, Rat, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
PP2A; Polyclonal Antibody; PP2A antibody (pAb); Protein Phosphatase 2A; PPP2R4; PR53; PTPA; anti-PP2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Peptide affinity purified IgG containing 30% glycerol, 0.035% sodium azide and 1mg/ml BSA. Sodium azide is highly toxic.
Applicable Applications for anti-PP2A antibody
Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 2-10ul per ChIP
WB: 1:250-1:2,000 dilution
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ChIP: 2-10ul per ChIP
WB: 1:250-1:2,000 dilution
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This PP2A antibody was raised against a peptide corresponding to amino acids 288-303 of human PP2A.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 ul of PP2A antibody or the equivalent amount of IgG as a negative control.)
Chromatin Immunoprecipitation (ChIP)
(PP2A antibody (pAb) tested by ChIP analysis. Chromatin IP performed using Swiss 3T3 Chromatin (approximately 5 million cells treated with anisomycin and fixed at 0.5 and 3 hours) using 2.5 ul of PP2A antibody or the equivalent amount of IgG as a negative control.)
Western Blot (WB)
(PP2A pAb tested by Western blot. Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).)
Western Blot (WB)
(PP2A pAb tested by Western blot. Nuclear extract of HeLa cells was probed with PP2A pAb (1:1,000 dilution).)
Related Product Information for anti-PP2A antibody
Short Description: Our PP2A antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human, Mouse and Rat samples, but it is predicted that it will react with a wide range of sample types.
Background: PP2A-Protein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers.
Background: PP2A-Protein Phosphatase 2A beta subunit is the catalytic subunit of protein phosphatase 2A. PP2A is involved in the regulation of a number of signaling pathways, and it is implicated in the negative control of cell growth and division. Mutations of PP2A occurs in many types of cancers.
Product Categories/Family for anti-PP2A antibody
NCBI and Uniprot Product Information
Molecular Weight
36kDa
Protein Family
Similar Products
Product Notes
The PP2A (Catalog #AAA388396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2A antibody (pAb) reacts with Human, Mouse, Rat, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Western Blot (WB). Applications Validated: ChIP: 2-10ul per ChIP WB: 1:250-1:2,000 dilution Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the PP2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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