Western Blot (WB)
(All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit PP2A alpha Polyclonal Antibody | anti-PPP2CA antibody
PP2A alpha Antibody (C-term)
IHC-P:~~1:50~100
Western Blot (WB)
(All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PPP2CA/B Antibody (F289) at 1:1000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)-Paraffin
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Background: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Protein type: Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: chromosome, pericentric region; cytosol; membrane; microtubule cytoskeleton; mitochondrion; nucleus; plasma membrane; protein phosphatase type 2A complex; spindle pole
Molecular Function: GABA receptor binding; metal ion binding; phosphoprotein phosphatase activity; protein binding; protein C-terminus binding
Biological Process: apoptosis; ceramide metabolic process; inactivation of MAPK activity; meiotic cell cycle; mesoderm development; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; negative regulation of cell growth; negative regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of cell differentiation; regulation of DNA replication; regulation of growth; regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; response to organic substance; RNA splicing; second-messenger-mediated signaling
Research Articles on PPP2CA
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Product Notes
The PPP2CA ppp2ca (Catalog #AAA9217170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2A alpha Antibody (C-term) reacts with Human; Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB:~~1:1000 IHC-P:~~1:50~100. Researchers should empirically determine the suitability of the PPP2CA ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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