Rabbit anti-Human PPP2CA Polyclonal Antibody | anti-PPP2CA antibody

PPP2CA Antibody

Cat. Num. AAA7006505

• Gene Names: PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha
• Reactivity: Human
• Applications: ELISA
• Purity: >95%,Protein G purified
• Synonyms: PPP2CA; Polyclonal Antibody; PPP2CA Antibody; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C; anti-PPP2CA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 309

• Applicable Applications for anti-PPP2CA antibody: ELISA (EIA)
• Immunogen: Recombinant human Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6110 / sc-130237 / sc-14020
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse liver tissue,Mouse kidney tissueAll lanes: PPP2CA antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 36,30 KDaObserved band size: 36,30 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7006505 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7006505 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PPP2CA antibody

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

NCBI and Uniprot Product Information

• NCBI GI #: 4506017
• NCBI GeneID: 5515
• NCBI Accession #: NP_002706.1
• NCBI GenBank Nucleotide #: NM_002715.2
• UniProt Accession #: P67775; P05323; P13197
• Molecular Weight: 29,735 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 catalytic subunit alpha
• NCBI Official Symbol: PPP2CA
• NCBI Official Synonym Symbols: RP-C; PP2Ac; PP2CA; PP2Calpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2CA
• UniProt Synonym Gene Names: PP2A-alpha; RP-C
• UniProt Entry Name: PP2AA_HUMAN

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: chromosome, pericentric region; cytosol; membrane; microtubule cytoskeleton; mitochondrion; nucleus; plasma membrane; protein phosphatase type 2A complex; spindle pole

Molecular Function: GABA receptor binding; metal ion binding; phosphoprotein phosphatase activity; protein binding; protein C-terminus binding

Biological Process: apoptosis; ceramide metabolic process; fibroblast growth factor receptor signaling pathway; gene expression; inactivation of MAPK activity; meiotic cell cycle; mesoderm development; mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; negative regulation of cell growth; negative regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of cell differentiation; regulation of DNA replication; regulation of growth; regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; response to organic substance; RNA splicing; second-messenger-mediated signaling