Rabbit anti-Human, Mouse PP1R14C Polyclonal Antibody | anti-PP1R14C antibody

PP1R14C, CT (PPP1R14C, KEPI, Protein phosphatase 1 regulatory subunit 14C, Kinase-enhanced PP1 inhibitor, PKC-potentiated PP1 inhibitory protein, Serologically defined breast cancer antigen NY-BR-81) (APC)

Cat. Num. AAA6336072

• Gene Names: PPP1R14C; KEPI; NY-BR-81; CPI17-like
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PP1R14C; Polyclonal Antibody; CT (PPP1R14C; KEPI; Protein phosphatase 1 regulatory subunit 14C; Kinase-enhanced PP1 inhibitor; PKC-potentiated PP1 inhibitory protein; Serologically defined breast cancer antigen NY-BR-81) (APC); PPP1R14C; Serologically defined breast cancer antigen NY-BR-81; anti-PP1R14C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PP1R14C. Species crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PP1R14C antibody: Immunohistochemistry (IHC), Western Blot (WB), FLISA
• Application Notes: IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PP1R14C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-163 amino acids from the C-terminal region of human PP1R14C.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PP1R14C Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the PP1R14C antibody detected the PP1R14C protein (arrow).)

Immunohistochemistry (IHC)

(PP1R14C Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PP1R14C Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PP1R14C antibody

The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1 (Liu et al., 2002).

Product Categories/Family for anti-PP1R14C antibody

Antibodies; Protein Kinases

References

1. Daskalow, K., et al. Anticancer Res. 30(5):1573-1578(2010). 2. Scholz, S.W., et al. Ann. Neurol. 65(5):610-614(2009). 3. Burgner, D., et al. PLoS Genet. 5 (1), E1000319 (2009). 4. Mungall, A.J., et al. Nature 425(6960):805-811(2003). 5. Liu, Q.R., et al. J. Biol. Chem. 277(15):13312-13320(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 19311006
• NCBI GeneID: 81706
• NCBI Accession #: NP_112211.1
• NCBI GenBank Nucleotide #: NM_030949.2
• UniProt Accession #: Q8TAE6; Q5VY83; Q96BB1; Q9H277
• Molecular Weight: 17,843 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14C
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 14C
• NCBI Official Symbol: PPP1R14C
• NCBI Official Synonym Symbols: KEPI; NY-BR-81; CPI17-like
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14C; kinase-enhanced PP1 inhibitor; kinase C-enhanced PP1 inhibitor; PKC-potentiated PP1 inhibitory protein; serologically defined breast cancer antigen NY-BR-81
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14C
• UniProt Gene Name: PPP1R14C
• UniProt Synonym Gene Names: KEPI
• UniProt Entry Name: PP14C_HUMAN

NCBI Description

The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1 (Liu et al., 2002 [PubMed 11812771]).[supplied by OMIM, Feb 2010]

Uniprot Description

KEPI: Inhibitor of PPP1CA. Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. Belongs to the PP1 inhibitor family.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 6q24.3-q25.3

Cellular Component: membrane; cytoplasm

Molecular Function: type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity