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Western Blot (WB) (Western blot analysis of PP15 expression in K562 (A), Hela (B) whole cell lysates.)

Rabbit PP15 Polyclonal Antibody | anti-NUTF2 antibody

Anti-PP15 Antibody

Gene Names
NUTF2; NTF2; PP15; NTF-2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
PP15; Polyclonal Antibody; Anti-PP15 Antibody; NTF2; Nuclear transport factor 2; NTF-2; Placental protein 15; anti-NUTF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PP15 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
127
Applicable Applications for anti-NUTF2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PP15. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of PP15 expression in K562 (A), Hela (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of PP15 expression in K562 (A), Hela (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PP15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of PP15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-NUTF2 antibody
Rabbit polyclonal antibody to PP15

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,478 Da
NCBI Official Full Name
Nuclear transport factor 2
NCBI Official Synonym Full Names
nuclear transport factor 2
NCBI Official Symbol
NUTF2
NCBI Official Synonym Symbols
NTF2; PP15; NTF-2
NCBI Protein Information
nuclear transport factor 2
UniProt Protein Name
Nuclear transport factor 2
UniProt Gene Name
NUTF2
UniProt Synonym Gene Names
NTF2; NTF-2; PP15
UniProt Entry Name
NTF2_HUMAN

NCBI Description

This gene encodes a cytosolic factor that facilitates protein transport into the nucleus. The encoded protein is required for nuclear import of the small Ras-like GTPase, Ran which is involved in numerous cellular processes. This protein also interacts with the nuclear pore complex glycoprotein p62. [provided by RefSeq, Apr 2016]

Uniprot Description

NUTF2: Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. Homodimer.

Protein type: Nuclear import

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol; nuclear outer membrane; nuclear pore

Molecular Function: protein binding; Ran GTPase binding; transporter activity

Biological Process: positive regulation of protein import into nucleus; protein export from nucleus; protein import into nucleus, translocation

Research Articles on NUTF2

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Product Notes

The NUTF2 nutf2 (Catalog #AAA8242736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PP15 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's PP15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the NUTF2 nutf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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