Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using DHRS2 antibody.)
Rabbit anti-Human, Mouse DHRS2 Polyclonal Antibody | anti-DHRS2 antibody
DHRS2 Antibody
Gene Names
DHRS2; HEP27; SDR25C1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DHRS2; Polyclonal Antibody; DHRS2 Antibody; 5430405K24Rik; Dehydrogenase/reductase (SDR family) member 2; Dehydrogenase/reductase member 2; Dehydrogenase/reductase SDR family member 2; Dehydrogenase/reductase SDR family member 2 mitochondria; Dicarbonyl reductase HEP27; Hep27; HEP27 protein; MGC143467; Protein D; SDR family member; SDR25C1; Short chain alcohol dehydrogenase family member; Short chain dehydrogenase/reductase family 25C member 1; anti-DHRS2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DHRS2 antibody detects endogenous levels of total DHRS2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
280
Applicable Applications for anti-DHRS2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion Matrix. Nucleus. Note: A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal.
Tissue Specificity
Widely expressed, with highest levels in liver and kidney, followed by heart, spleen, skeletal muscle and placenta. In hemopoietic cells, expressed in dendritic cells, but not in monocytes, macrophages, granulocytes, nor in B and T lymphocytes.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using DHRS2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using DHRS2 antibody.)
Immunofluorescene (IF)
(MBS9605441 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605441 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-DHRS2 antibody
Function: Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3, 4-Hexanedione, 2, 3-Heptanedione and 1-Phenyl-1, 2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.
Subunit Structure: Directly interacts with MDM2; this interaction occurs in the nucleus and does not target DHRS2 to degradation.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Subunit Structure: Directly interacts with MDM2; this interaction occurs in the nucleus and does not target DHRS2 to degradation.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kD
Predicted: 30 kDa
Predicted: 30 kDa
NCBI Official Full Name
dehydrogenase/reductase SDR family member 2, mitochondrial isoform 2
NCBI Official Synonym Full Names
dehydrogenase/reductase 2
NCBI Official Symbol
DHRS2
NCBI Official Synonym Symbols
HEP27; SDR25C1
NCBI Protein Information
dehydrogenase/reductase SDR family member 2, mitochondrial
UniProt Protein Name
Dehydrogenase/reductase SDR family member 2, mitochondrial
Protein Family
UniProt Gene Name
DHRS2
UniProt Synonym Gene Names
SDR25C1
NCBI Description
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family, which has over 46,000 members. Members of this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative promoter use and alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
Uniprot Description
Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.
Research Articles on DHRS2
Similar Products
Product Notes
The DHRS2 dhrs2 (Catalog #AAA9605441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DHRS2 dhrs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
