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Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in human skeletal muscle with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit Porin/VDAC1 Polyclonal Antibody | anti-VDAC1 antibody
Anti-Porin/VDAC1 Antibody, Rabbit Polyclonal
Gene Names
VDAC1; PORIN; VDAC-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Porin/VDAC1; Polyclonal Antibody; Anti-Porin/VDAC1 Antibody; Rabbit Polyclonal; VDAC1 Antibody; Rabbit PAb; Antigen Affinity Purified; voltage-dependent anion channel 1; Anti-PORIN Antibody; Anti-VDAC-1 Antibody; anti-VDAC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Porin/VDAC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-VDAC1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:3000
IP: 1-4uL/mg of lysate
IHC-P: 1:500-1:3000
IP: 1-4uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the center region of the Human VDAC1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human VDAC1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in human skeletal muscle with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in human skeletal muscle with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining VDAC1 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Western Blot (WB)
(Anti-VDAC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:31 kDa Observed band size:31 kDa)
Western Blot (WB)
(Anti-VDAC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:31 kDa Observed band size:31 kDa)
Immunoprecipitation (IP)
(VDAC1 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-VDAC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-VDAC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 35 kDa)
Immunoprecipitation (IP)
(VDAC1 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-VDAC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-VDAC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 35 kDa)
Related Product Information for anti-VDAC1 antibody
VDACs, or Voltage-Dependent Anion Channels are the most abundant pore-forming proteins of the Mitochondrial Outer Membrane (MOM). High conserved through evolution, in mammals three distinct genes encode for three different VDAC isoforms, namely VDAC1, VDAC2, and VDAC3. VDAC1 serves as the main gate in the MOM for metabolites, such as ATP/ADP and NAD+/NADH, but also Krebs cycle's intermediates, cholesterol and glutamate. Furthermore, by interaction with many cytosolic enzymes, such as Hexokinases (HK), Glucokinase (GK), and Creatine Kinase (CK), VDAC1 provides the ATP source essential for enzyme's activity. In addition, VDAC1 represents a hub protein that interacts with over 150 other proteins including phosphorylated tau, Abeta, and gamma-secretase, and participates in their toxicity. Therefore, regulation of VDAC1 is crucial not only for metabolic functions of mitochondria, but also for cell survival.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31kDa
NCBI Official Full Name
voltage-dependent anion-selective channel protein 1
NCBI Official Synonym Full Names
voltage-dependent anion channel 1
NCBI Official Symbol
VDAC1
NCBI Official Synonym Symbols
PORIN; VDAC-1
NCBI Protein Information
voltage-dependent anion-selective channel protein 1; porin 31HL; porin 31HM; plasmalemmal porin; outer mitochondrial membrane protein porin 1
UniProt Protein Name
Voltage-dependent anion-selective channel protein 1
Protein Family
UniProt Gene Name
VDAC1
UniProt Synonym Gene Names
VDAC; VDAC-1; hVDAC1
UniProt Entry Name
VDAC1_HUMAN
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Product Notes
The VDAC1 vdac1 (Catalog #AAA8101102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Porin/VDAC1 Antibody, Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Porin/VDAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:500-1:3000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the VDAC1 vdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Porin/VDAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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