Goat anti-Human, Rat PLEKHB1 Polyclonal Antibody | anti-PLEKHB1 antibody

PLEKHB1 (Pleckstrin homology domain containing, family B (evectins) member 1, KPL1, PHR1, PHRET1, PH domain containing protein in retina 1, PH domain containing, retinal 1)

Cat. Num. AAA620754

• Gene Names: PLEKHB1; KPL1; PHR1; PHRET1
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: PLEKHB1; Polyclonal Antibody; PLEKHB1 (Pleckstrin homology domain containing; family B (evectins) member 1; KPL1; PHR1; PHRET1; PH domain containing protein in retina 1; PH domain containing; retinal 1); Anti -PLEKHB1 (Pleckstrin homology domain containing; anti-PLEKHB1 antibody

• Host: Goat
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Recognizes isoforms a and b of PLEKHB1 at ~25kD. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PLEKHB1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:16000; Western Blot: 1-3ug/ml. ~25kD band observed in rat brain lysates (calculated MW of 25.2kD according to rat NP_742030.1 (Evectin-1) and of 27.2kD according to human NP_067023.1).
• Immunogen: Synthetic peptide with sequence PPDSALESPFEE-C, from the N Terminus of human PLEKHB1(NP_067023.1; NP_001123506.1; NP_001123505.1; NP_001123507.1; NP_001123508.1).
• Positive Control: Rat Brain lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(MBS620754 (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-PLEKHB1 antibody

Antibodies; Abs to Motor Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 14165543
• NCBI GeneID: 58473
• UniProt Accession #: Q59EU5; Q9UF11
• Molecular Weight: 23,585 Da
• NCBI Official Full Name: PLEKHB1 protein
• NCBI Official Synonym Full Names: pleckstrin homology domain containing, family B (evectins) member 1
• NCBI Official Symbol: PLEKHB1
• NCBI Official Synonym Symbols: KPL1; PHR1; PHRET1
• NCBI Protein Information: pleckstrin homology domain-containing family B member 1; evectin-1; OTTHUMP00000237624; OTTHUMP00000237625; OTTHUMP00000237627; OTTHUMP00000237628; OTTHUMP00000237629; OTTHUMP00000237631; OTTHUMP00000237632; OTTHUMP00000237633; OTTHUMP00000237634; OTTHUMP00000237636; OTTHUMP00000237637; OTTHUMP00000237638; OTTHUMP00000237639; OTTHUMP00000237640; PH domain containing, retinal 1; PH domain-containing family B member 1; PH domain containing protein in retina 1; PH domain-containing protein in retina 1; pleckstrin homology domain retinal protein 1
• UniProt Protein Name: Pleckstrin homology domain containing, family B (Evectins) member 1 variant
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Entry Name: Q59EU5_HUMAN

Uniprot Description

PLEKHB1: Required for proper localization of retinogeniculate projections but not for eye-specific segregation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 11q13.5-q14.1

Cellular Component: cytoplasm; integral to membrane

Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; protein homodimerization activity

Biological Process: regulation of cell differentiation; multicellular organismal development; phototransduction