Rabbit anti-Human PON1 Polyclonal Antibody | anti-PON1 antibody

PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP)

Cat. Num. AAA6335877

• Gene Names: PON1; ESA; PON; MVCD5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PON1; Polyclonal Antibody; ID (PON1; PON; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; K-45; Serum aryldialkylphosphatase 1) (Azide free) (HRP); Serum aryldialkylphosphatase 1; anti-PON1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PON1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PON1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 118-145 in the central region of human PON1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PON1 (arrow) in 293 cell lysates (2ug/lane) using MBS649722 either nontransfected (Lane 1) or transiently transfected with the PON1 gene (Lane 2).)

Western Blot (WB)

(Western Blot analysis of lysate from human blood plasma tissue lysate (35ug), MBS649722 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS649722 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649722 for immunohistochemistry.)

Product Categories/Family for anti-PON1 antibody

Antibodies; Enzymes; Phosphatase

References

1. Ergun, M.A., et al. Biochem. Genet. (2010). In press: 2. Cagirci, G., et al. J. Heart Valve Dis. 19(4):453-458(2010). 3. Mendonca, M.I., et al. Rev Port Cardiol 29(4):571-580(2010). 4. Hashemi, M., et al. Genet. Mol. Res. 9(3):1735-1741(2010). 5. Martinez, C., et al. BMC Neurol 10, 71 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 19923106
• NCBI GeneID: 5444
• NCBI Accession #: NP_000437.3
• NCBI GenBank Nucleotide #: NM_000446.5
• UniProt Accession #: P27169; Q16052; Q6B0J6; Q9UCB1; B2RA40
• Molecular Weight: 40kDa
• NCBI Official Full Name: serum paraoxonase/arylesterase 1
• NCBI Official Synonym Full Names: paraoxonase 1
• NCBI Official Symbol: PON1
• NCBI Official Synonym Symbols: ESA; PON; MVCD5
• NCBI Protein Information: serum paraoxonase/arylesterase 1; K-45; PON 1; esterase A; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1
• UniProt Protein Name: Serum paraoxonase/arylesterase 1
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON1
• UniProt Synonym Gene Names: PON; PON 1
• UniProt Entry Name: PON1_HUMAN

Uniprot Description

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: EC 3.1.8.1; EC 3.1.1.2; Lipid-binding; Secreted, signal peptide; Motility/polarity/chemotaxis; EC 3.1.1.81; Hydrolase; Phosphatase (non-protein); Secreted

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region

Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity

Biological Process: response to external stimulus; response to nutrient levels; dephosphorylation; response to toxin; organophosphate catabolic process; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5