Rat Serum paraoxonase/arylesterase 1 ELISA Kit | Pon1 elisa kit

Rat Serum paraoxonase/arylesterase 1 ELISA Kit

Cat. Num. AAA764159

• Gene Names: PON1; ESA; PON; MVCD5
• Reactivity: Rat
• Synonyms: Serum paraoxonase/arylesterase 1; Rat Serum paraoxonase/arylesterase 1 ELISA Kit; Pon1/ESA/K-45/A-esterase 1/Aromatic esterase 1/arylesterase B-type/ESAesterase A/K-45/MVCD5/paraoxonase 1/PON 1/PONparaoxonase B-type/serum aryldiakylphosphatase/Serum aryldialkylphosphatase 1/serum paraoxonase/arylesterase 1; Pon1 elisa kit

• Reactivity: Rat
• Sequence Length: 355

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Pon1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Pon1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Pon1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Pon1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Pon1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 19923106
• NCBI GeneID: 5444
• NCBI Accession #: NP_000437.3
• NCBI GenBank Nucleotide #: NM_000446.5
• Molecular Weight: 39,731 Da
• NCBI Official Full Name: serum paraoxonase/arylesterase 1
• NCBI Official Synonym Full Names: paraoxonase 1
• NCBI Official Symbol: PON1
• NCBI Official Synonym Symbols: ESA; PON; MVCD5
• NCBI Protein Information: serum paraoxonase/arylesterase 1
• UniProt Protein Name: Serum paraoxonase/arylesterase 1
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON1
• UniProt Synonym Gene Names: PON; A-esterase 1
• UniProt Entry Name: PON1_HUMAN

NCBI Description

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

Uniprot Description

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: Secreted; EC 3.1.1.2; Phosphatase (non-protein); EC 3.1.1.81; Hydrolase; Secreted, signal peptide; Lipid-binding; Motility/polarity/chemotaxis; EC 3.1.8.1

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular region; extracellular space; intracellular membrane-bound organelle

Molecular Function: aryldialkylphosphatase activity; arylesterase activity; calcium ion binding; phospholipid binding; protein homodimerization activity

Biological Process: aromatic compound catabolic process; carboxylic acid catabolic process; dephosphorylation; organophosphate catabolic process; phosphatidylcholine metabolic process; positive regulation of binding; positive regulation of transporter activity; response to external stimulus; response to nutrient levels; response to toxin

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5