Rabbit POMGNT1 Polyclonal Antibody | anti-POMGNT1 antibody

POMGNT1 Polyclonal Antibody

Cat. Num. AAA3009332

• Gene Names: POMGNT1; MEB; RP76; GNTI.2; LGMD2O; GnT I.2; LGMDR15; MGAT1.2; gnT-I.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POMGNT1; Polyclonal Antibody; POMGNT1 Polyclonal Antibody; OMGnT1; GnT I.2; MGAT1.2; UNQ746/PRO1475; anti-POMGNT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POMGNT1 polyclonal antibody detects endogenous levels of POMGNT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 748

• Applicable Applications for anti-POMGNT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human POMGNT1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using POMGNT1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using POMGNT1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using POMGNT1 antibody at dilution of 1:100 (x40 lens).)

Related Product Information for anti-POMGNT1 antibody

The product of the POMGNT1 gene, protein O-mannose beta-1, 2-N-acetylglucosaminyltransferase, participates in O-mannosyl glycan synthesis. POMGnT1 is an N(in)/C(out) (type II) membrane protein localized in the medial-Golgi that initiates the conversion of high mannose N-glycans to complex N-glycans. Specifically, POMGnT1 is a glycosylation enzyme that participates in the synthesis of O-mannosyl glycan, a laminin-binding ligand of alpha-dystroglycan that is rarely synthesized in mammals. Mutations in the POMGNT1 gene cause muscle-eye-brain disease (MEB), an autosomal recessive disorder characterized by congenital muscular dystrophy, ocular abnormalities and lissencephaly.

NCBI and Uniprot Product Information

• NCBI GI #: 344313163
• NCBI GeneID: 55624
• NCBI Accession #: NP_001230695
• NCBI GenBank Nucleotide #: NP_001230695.1
• UniProt Accession #: Q8WZA1
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-)
• NCBI Official Symbol: POMGNT1
• NCBI Official Synonym Symbols: MEB; RP76; GNTI.2; LGMD2O; GnT I.2; LGMDR15; MGAT1.2; gnT-I.2
• NCBI Protein Information: protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
• UniProt Protein Name: Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
• Protein Family: Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
• UniProt Gene Name: POMGNT1
• UniProt Synonym Gene Names: MGAT1.2; POMGnT1; GnT I.2
• UniProt Entry Name: PMGT1_HUMAN

NCBI Description

This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014]