Rabbit MGAT1 Polyclonal Antibody | anti-MGAT1 antibody

MGAT1 Antibody

Cat. Num. AAA9611410

• Gene Names: POMGNT1; MEB; RP76; GNTI.2; LGMD2O; GnT I.2; LGMDR15; MGAT1.2; gnT-I.2
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MGAT1; Polyclonal Antibody; MGAT1 Antibody; anti-MGAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MGAT1 Antibody detects endogenous levels of MGAT1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 748

• Applicable Applications for anti-MGAT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MGAT1.
• Tissue Specificity: Constitutively expressed. An additional weaker band is also detected in spinal cord, lymph node, and trachea. Expressed especially in astrocytes. Also expressed in immature and mature neurons.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Golgi Apparatus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Myeloma cells, using MGAT1 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using MGAT1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MGAT1 antibody

Description: Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins (PubMed:11709191, PubMed:27493216). Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties (PubMed:11709191, PubMed:27493216). Is specific for alpha linked terminal mannose and does not have MGAT3, MGAT4, MGAT5, MGAT7 or MGAT8 activity.

Subunit Structure: Interacts with DAG1 (via O-linked mannose moiety) (PubMed:27493216). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes (PubMed:17034757).

Similarity: Amino acid residues between 299-311 are important for both protein expression and enzymatic activity. The minimal catalytic domain is located between positions 299-651. Single amino acid substitutions in the stem domain from MEB patients abolished the activity of the membrane-bound form but not the soluble form. This suggests that the stem domain of the soluble form is unnecessary for activity, but that some amino acids play a crucial role in the membrane-bound form. The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein. Belongs to the glycosyltransferase 13 family.

NCBI and Uniprot Product Information

• NCBI GI #: 344313163
• NCBI GeneID: 55624
• NCBI Accession #: NP_001230695
• NCBI GenBank Nucleotide #: NP_001230695.1
• UniProt Accession #: Q8WZA1
• Molecular Weight: Observed: 55 kDa; Predicted: 76 kDa
• NCBI Official Full Name: protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-)
• NCBI Official Symbol: POMGNT1
• NCBI Official Synonym Symbols: MEB; RP76; GNTI.2; LGMD2O; GnT I.2; LGMDR15; MGAT1.2; gnT-I.2
• NCBI Protein Information: protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
• UniProt Protein Name: Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
• UniProt Gene Name: POMGNT1
• UniProt Synonym Gene Names: MGAT1.2; POMGnT1; GnT I.2
• UniProt Entry Name: PMGT1_HUMAN

NCBI Description

This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014]