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Western Blot (WB) (Western blotAll lanes: Polypeptide N-acetylgalactosaminyltransferase 14 antibody at 2ug/mlLane 1:rat gonad tissueLane 2:mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 65,61,62 kDaObserved band size: 64 kDa)

Rabbit Polypeptide N-acetylgalactosaminyltransferase 14 Polyclonal Antibody | anti-GALNT14 antibody

Rabbit anti-human Polypeptide N-acetylgalactosaminyltransferase 14 polyclonal Antibody(GALNT14)

Gene Names
GALNT14; GALNT15; GalNac-T10; GalNac-T14
Reactivity
Human, mouse, rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Polypeptide N-acetylgalactosaminyltransferase 14; Polyclonal Antibody; Rabbit anti-human Polypeptide N-acetylgalactosaminyltransferase 14 polyclonal Antibody(GALNT14); Polypeptide GalNAc transferase 14; GalNAc-T14; pp-GaNTase 14; Protein-UDP acetylgalactosaminyltransferase 14; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 14; GALNT14; anti-GALNT14 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
557
Applicable Applications for anti-GALNT14 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Polypeptide N-acetylgalactosaminyltransferase 14 protein(1-552aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68509 / sc-68510
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: Polypeptide N-acetylgalactosaminyltransferase 14 antibody at 2ug/mlLane 1:rat gonad tissueLane 2:mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 65,61,62 kDaObserved band size: 64 kDa)

Western Blot (WB) (Western blotAll lanes: Polypeptide N-acetylgalactosaminyltransferase 14 antibody at 2ug/mlLane 1:rat gonad tissueLane 2:mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 65,61,62 kDaObserved band size: 64 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS7005840 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine using MBS7005840 at dilution 1:100)
Related Product Information for anti-GALNT14 antibody
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays activity toward mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). May be involved in O-glycosylation in kidney.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,588 Da
NCBI Official Full Name
polypeptide N-acetylgalactosaminyltransferase 14 isoform 2
NCBI Official Synonym Full Names
polypeptide N-acetylgalactosaminyltransferase 14
NCBI Official Symbol
GALNT14
NCBI Official Synonym Symbols
GALNT15; GalNac-T10; GalNac-T14
NCBI Protein Information
polypeptide N-acetylgalactosaminyltransferase 14
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 14
UniProt Gene Name
GALNT14
UniProt Synonym Gene Names
GalNAc-T14; pp-GaNTase 14
UniProt Entry Name
GLT14_HUMAN

NCBI Description

This gene encodes a Golgi protein which is a member of the polypeptide N-acetylgalactosaminyltransferase (ppGalNAc-Ts) protein family. These enzymes catalyze the transfer of N-acetyl-D-galactosamine (GalNAc) to the hydroxyl groups on serines and threonines in target peptides. The encoded protein has been shown to transfer GalNAc to large proteins like mucins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

GALNT14: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Displays activity toward mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). May be involved in O-glycosylation in kidney. Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - O-glycan biosynthesis; Transferase; EC 2.4.1.41; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: carbohydrate binding; metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: cellular protein metabolic process; O-glycan processing; post-translational protein modification; protein amino acid O-linked glycosylation

Research Articles on GALNT14

Similar Products

Product Notes

The GALNT14 galnt14 (Catalog #AAA7005840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Polypeptide N-acetylgalactosaminyltransferase 14 polyclonal Antibody(GALNT14) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Polypeptide N-acetylgalactosaminyltransferase 14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the GALNT14 galnt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Polypeptide N-acetylgalactosaminyltransferase 14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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