Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cells.)
Rabbit anti-Human Polymerase DNA Directed Delta 1 Polyclonal Antibody | anti-POLd antibody
Polymerase DNA Directed Delta 1 (POLd) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant POLd, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant POLd, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded lung tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
Uniprot Description
POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.
Protein type: DNA repair, damage; DNA replication; EC 2.7.7.7; Hydrolase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cytosol; delta DNA polymerase complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleotide-excision repair complex; nucleus
Molecular Function: 3'-5'-exodeoxyribonuclease activity; 4 iron, 4 sulfur cluster binding; chromatin binding; damaged DNA binding; DNA binding; DNA-directed DNA polymerase activity; metal ion binding; nucleotide binding; protein binding
Biological Process: base-excision repair, gap-filling; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA ligation; DNA repair; DNA replication; DNA replication proofreading; DNA synthesis involved in DNA repair; fatty acid homeostasis; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; response to UV; telomere maintenance; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair
Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome
Research Articles on POLd
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Product Notes
The POLd pold1 (Catalog #AAA2032450) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polymerase DNA Directed Delta 1 (POLd) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Polymerase DNA Directed Delta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the POLd pold1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Polymerase DNA Directed Delta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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