Rabbit POLR3F Polyclonal Antibody | anti-POLR3F antibody

POLR3F Polyclonal Antibody

Cat. Num. AAA3006415

• Gene Names: POLR3F; C34; RPC6; RPC39
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR3F; Polyclonal Antibody; POLR3F Polyclonal Antibody; DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; RNA polymerase III 39 kDa subunit; RPC39; Pol III RPC39; anti-POLR3F antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR3F polyclonal antibody detects endogenous levels of POLR3F protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 275

• Applicable Applications for anti-POLR3F antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human POLR3F.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of POLR3F polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-POLR3F antibody

Eukaryotes produce three distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S and 5.8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes.

NCBI and Uniprot Product Information

• NCBI GI #: 542133141
• NCBI GeneID: 10621
• NCBI Accession #: NP_001269455.1
• NCBI GenBank Nucleotide #: NP_001269455.1
• UniProt Accession #: Q9H1D9
• Molecular Weight: ~ 39kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC6 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit F
• NCBI Official Symbol: POLR3F
• NCBI Official Synonym Symbols: C34; RPC6; RPC39
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39
• UniProt Entry Name: RPC6_HUMAN

NCBI Description

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Protein type: Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter