Rabbit POLR3F Polyclonal Antibody | anti-POLR3F antibody

POLR3F Antibody

Cat. Num. AAA151394

• Gene Names: POLR3F; RPC6; RPC39
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: POLR3F Antibody is affinity chromatography purified via peptide column.
• Synonyms: POLR3F; Polyclonal Antibody; POLR3F Antibody; RPC6; RPC39; DNA-directed RNA polymerase III subunit RPC6; DNA-directed RNA polymerase III subunit F; RNA polymerase III subunit C6; polymerase (RNA) III (DNA directed) polypeptide F; 39 kDa; anti-POLR3F antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: POLR3F Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 316

• Applicable Applications for anti-POLR3F antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: POLR3F antibody was raised against a 21 amino acid synthetic peptide from near the amino terminus of human POLR3F.
• Buffer: POLR3F Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: POLR3F antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of POLR3F in human brain tissue with POLR3F antibody at 20 μg/mL.)

Related Product Information for anti-POLR3F antibody

POLR3F Antibody: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 10636565
• NCBI GeneID: 10621
• UniProt Accession #: Q9H1D9; O15319; A8K4C7
• Molecular Weight: 35,684 Da
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
• NCBI Official Symbol: POLR3F
• NCBI Official Synonym Symbols: RPC6; RPC39
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC6
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39
• UniProt Entry Name: RPC6_HUMAN

NCBI Description

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter