Rabbit POLR3F Polyclonal Antibody | anti-POLR3F antibody

POLR3F Antibody

Cat. Num. AAA9406659

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: POLR3F; Polyclonal Antibody; POLR3F Antibody; RNA polymerase III polypeptide F; RNA polymerase subunit C6; RPC6; RPC39; anti-POLR3F antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 316

• Applicable Applications for anti-POLR3F antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 21 amino acid peptide from near the amino terminus of human POLR3F.
• Target Name: POLR3F
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of POLR3F in human brain tissue with POLR3F antibody at 2.5 ug/mL.)

Related Product Information for anti-POLR3F antibody

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Product Categories/Family for anti-POLR3F antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10636565
• UniProt Accession #: Q9H1D9; O15319; A8K4C7
• Molecular Weight: 35,684 Da
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC6
• UniProt Gene Name: POLR3F
• UniProt Synonym Gene Names: RNA polymerase III subunit C6; RPC39
• UniProt Entry Name: RPC6_HUMAN

Uniprot Description

POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.

Protein type: Transcription initiation complex; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter