Rabbit c-TAK1 Polyclonal Antibody | anti-c-TAK1 antibody

c-TAK1 (P8) Polyclonal Antibody

Cat. Num. AAA3001964

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: c-TAK1; Polyclonal Antibody; c-TAK1 (P8) Polyclonal Antibody; C-TAK1; Cdc25C associated protein kinase 1; Cdc25C-associated protein kinase 1; cTAK1; ELKL motif kinase 2; EMK-2; Emk2; ETK 1; KIAA4230; KP78; MAP microtubule affinity regulating kinase 3; MAP/microtubule affinity-regulating kinase 3; Mark3; MARK3; Par 1a; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine threonine protein kinase p78; Serine/threonine-protein kinase p78; SerThr protein kinase PAR 1; CTAK1; anti-c-TAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: c-TAK1 (P8) polyclonal antibody detects endogenous levels of C-TAK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 753

• Applicable Applications for anti-c-TAK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human c-TAK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of c-TAK1 (P8) pAb at 1:1000 dilutionLane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of c-TAK1 (P8) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-c-TAK1 antibody

c-TAK1 (Cdc25C associated protein kinase) phosphorylates Cdc25C on Ser 216 and is ubiquitously expressed in various human tissue and cell lines. C-TAK1 is distinct from Chk1, which also phosphorylates Cdc25C on Ser 216 in response to DNA damage. Phosphorylation of Cdc25C allows for the preferential binding of 14-3-3 proteins, subsequently retaining Cdc25C in the cytoplasm. Thus, the binding of 14-3-3 proteins prevents Cdc25C from dephosphorylating Cdc2 in the nucleus, thereby controlling the entry of the cells into mitosis. It is suggested that C-TAK1 mediates the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 193083125
• NCBI Accession #: NP_001122390.1
• NCBI GenBank Nucleotide #: NP_001122390.1
• UniProt Accession #: P27448
• Molecular Weight: ~ 87kDa
• NCBI Official Full Name: MAP/microtubule affinity-regulating kinase 3 isoform a
• UniProt Protein Name: MAP/microtubule affinity-regulating kinase 3
• UniProt Gene Name: MARK3
• UniProt Synonym Gene Names: CTAK1; EMK2; cTAK1; EMK-2; Par-1a
• UniProt Entry Name: MARK3_HUMAN

Uniprot Description

MARK3: an ubiquitous serine/threonine kinase of the CAMKL family. Phosphorylates Cdc25C on serine 216 and promotes 14-3-3 protein binding. A positive regulator of Wnt signalling. Ubiquitously expressed in human tissues and cell lines. Seven alternatively spliced isoforms have been reported.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; MARK subfamily

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: protein amino acid phosphorylation