Rabbit anti-Human POLR3C Polyclonal Antibody | anti-POLR3C antibody

POLR3C, ID (POLR3C, DNA-directed RNA polymerase III subunit RPC3, DNA-directed RNA polymerase III subunit C, RNA polymerase III 62kD subunit) (FITC)

Cat. Num. AAA6335788

• Gene Names: POLR3C; RPC3; RPC62
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR3C; Polyclonal Antibody; ID (POLR3C; DNA-directed RNA polymerase III subunit RPC3; DNA-directed RNA polymerase III subunit C; RNA polymerase III 62kD subunit) (FITC); RNA polymerase III 62kD subunit; anti-POLR3C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-POLR3C antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human POLR3C.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POLR3C Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR3C antibody detected the POLR3C protein (arrow).)

Western Blot (WB)

(Western blot analysis of POLR3C (arrow) using rabbit polyclonal POLR3C Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR3C gene.)

Related Product Information for anti-POLR3C antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Product Categories/Family for anti-POLR3C antibody

Antibodies; Enzymes; Polymerase

References

Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009). Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002). Kuwana, M., et al. Arthritis Rheum. 46(10):2742-2747(2002). Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999). Hsieh, Y.J., et al. Mol. Cell. Biol. 19(7):4944-4952(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 21359969
• NCBI GeneID: 10623
• NCBI Accession #: NP_006459.3
• NCBI GenBank Nucleotide #: NM_006468.6
• UniProt Accession #: Q9BUI4; O15317; Q9Y3R6
• Molecular Weight: 60,612 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC3
• NCBI Official Synonym Full Names: polymerase (RNA) III (DNA directed) polypeptide C (62kD)
• NCBI Official Symbol: POLR3C
• NCBI Official Synonym Symbols: RPC3; RPC62
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC3; RNA polymerase III subunit C3; RNA polymerase III 62 kDa subunit; DNA-directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC3
• UniProt Gene Name: POLR3C
• UniProt Synonym Gene Names: RNA polymerase III subunit C3; RPC62
• UniProt Entry Name: RPC3_HUMAN

Uniprot Description

POLR3C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.

Protein type: Transferase; Transcription initiation complex; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; nucleus; cytosol

Molecular Function: DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; regulation of transcription from RNA polymerase III promoter; positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter