Rabbit MEK2 Polyclonal Antibody | anti-MEK2 antibody

MEK2 (Phospho-Thr394) Antibody

Cat. Num. AAA9503093

• Gene Names: MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MEK2; Polyclonal Antibody; MEK2 (Phospho-Thr394) Antibody; MAP2K2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MKK2; PRKMK2; anti-MEK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: MEK2 (Phospho-Thr394) Antibody detects endogenous levels of MEK2 only when phosphorylated at Thr394.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-MEK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Immunogen: The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394.
• Immunogen Range: 261-310
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2 (Phospho-Thr394) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from ovary cancer, using MEK2 (Phospho-Thr394) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 13489054
• NCBI GeneID: 5605
• NCBI Accession #: NP_109587.1
• NCBI GenBank Nucleotide #: NM_030662.3
• UniProt Accession #: P36507
• Molecular Weight: 44 kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 2
• NCBI Official Symbol: MAP2K2
• NCBI Official Synonym Symbols: CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 2
• UniProt Gene Name: MAP2K2
• UniProt Synonym Gene Names: MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases ().