Rabbit anti-Human POLR2E Polyclonal Antibody | anti-POLR2E antibody

POLR2E, NT (POLR2E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerase II 23kD polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4) (PE)

Cat. Num. AAA6335718

• Gene Names: POLR2E; RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR2E; Polyclonal Antibody; NT (POLR2E; DNA-directed RNA polymerases I; II; and III subunit RPABC1; DNA-directed RNA polymerase II 23kD polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4) (PE); XAP4; anti-POLR2E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-POLR2E antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of human POLR2E.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR2E Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the POLR2E antibody detected the POLR2E protein (arrow).)

Related Product Information for anti-POLR2E antibody

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Product Categories/Family for anti-POLR2E antibody

Antibodies; DNA Replication; Repair

References

Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Cloutier, P., et al. Methods 48(4):381-386(2009). Michiels, S., et al. Carcinogenesis 30(5):763-768(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Le, T.T., et al. J. Biochem. 138(3):215-224(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 14589951
• NCBI GeneID: 5434
• NCBI Accession #: NP_002686.2
• NCBI GenBank Nucleotide #: NM_002695.3
• UniProt Accession #: P19388; O43380; Q6PIH5; Q9BT06; B2R6L4; D6W5Y1
• Molecular Weight: 24,551 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• NCBI Official Symbol: POLR2E
• NCBI Official Synonym Symbols: RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC1; RPB5 homolog; DNA-directed RNA polymerase II subunit E; RNA polymerases I, II, and III subunit ABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polyp
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• UniProt Gene Name: POLR2E
• UniProt Synonym Gene Names: RNA polymerases I, II, and III subunit ABC1
• UniProt Entry Name: RPAB1_HUMAN

NCBI Description

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; nucleus; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of gene expression, epigenetic; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase III promoter; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter