Rabbit anti-Human, Mouse POLR2C Polyclonal Antibody | anti-POLR2C antibody

POLR2C (C-Term) Rabbit pAb

Cat. Num. AAA8555819

• Gene Names: POLR2C; RPB3; RPB31; hRPB33; hsRPB3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: POLR2C; Polyclonal Antibody; POLR2C (C-Term) Rabbit pAb; DNA-directed RNA polymerase II subunit RPB3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; DNA-directed RNA polymerase II 33 kDa polypeptide; RPB33; DNA-directed RNA polymerase II subunit C; RPB31; anti-POLR2C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in PBS with 0.09% (W/V) Sodium Azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 275

• Applicable Applications for anti-POLR2C antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:25
• Immunogen: This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C.
• Cellular Localization: DNA-Directed RNA Polymerase, Nucleus

NCBI and Uniprot Product Information

• NCBI GI #: 3915850
• NCBI GeneID: 5432
• UniProt Accession #: P19387
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB3
• NCBI Official Synonym Full Names: RNA polymerase II subunit C
• NCBI Official Symbol: POLR2C
• NCBI Official Synonym Symbols: RPB3; RPB31; hRPB33; hsRPB3
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB3
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB3
• UniProt Gene Name: POLR2C
• UniProt Synonym Gene Names: RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB33
• UniProt Entry Name: RPB3_HUMAN

NCBI Description

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

Protein type: DNA-binding; Nucleotide Metabolism - purine; Transcription initiation complex; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 16q13-q21

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein dimerization activity; DNA binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; nucleotide-excision repair; somatic stem cell maintenance; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair