Bovine ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit | ALOX12 elisa kit

Bovine ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit

Cat. Num. AAA7606195

• Gene Names: ALOX12; LOG12; 12-LOX; 12S-LOX
• Reactivity: Bovine
• Synonyms: ALOX12 (Arachidonate-12-Lipoxygenase); Bovine ALOX12 (Arachidonate-12-Lipoxygenase) ELISA Kit; ALOX12 elisa kit

• Reactivity: Bovine
• Sequence Length: 112

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX12 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ALOX12 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ALOX12 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ALOX12 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALOX12 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 219393
• NCBI GeneID: 239
• Molecular Weight: 75,694 Da
• NCBI Official Full Name: arachidonate 12-lipoxygenase, partial
• NCBI Official Synonym Full Names: arachidonate 12-lipoxygenase, 12S type
• NCBI Official Symbol: ALOX12
• NCBI Official Synonym Symbols: LOG12; 12-LOX; 12S-LOX
• NCBI Protein Information: arachidonate 12-lipoxygenase, 12S-type
• UniProt Protein Name: Arachidonate 12-lipoxygenase, 12S-type
• Protein Family: Arachidonate 12-lipoxygenase
• UniProt Gene Name: ALOX12
• UniProt Synonym Gene Names: 12LO; LOG12; 12S-LOX; 12S-lipoxygenase

Uniprot Description

ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.

Protein type: Apoptosis; EC 1.13.11.31; Lipid Metabolism - arachidonic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytosol; membrane; sarcolemma

Molecular Function: arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; lipoxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding

Biological Process: arachidonic acid metabolic process; cell motility; fatty acid oxidation; hepoxilin biosynthetic process; hepoxilin metabolic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation