Rabbit anti-Human POLDIP3 Polyclonal Antibody | anti-POLDIP3 antibody

POLDIP3, NT (POLDIP3, KIAA1649, PDIP46, Polymerase delta-interacting protein 3, 46kD DNA polymerase delta interaction protein, S6K1 Aly/REF-like target)

Cat. Num. AAA6002300

• Gene Names: POLDIP3; SKAR; PDIP46
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: POLDIP3; Polyclonal Antibody; NT (POLDIP3; KIAA1649; PDIP46; Polymerase delta-interacting protein 3; 46kD DNA polymerase delta interaction protein; S6K1 Aly/REF-like target); Anti -POLDIP3; anti-POLDIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-POLDIP3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: POLDIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human POLDIP3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POLDIP3 Antibody (N-term) western blot analysis in Jurkat,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the POLDIP3 Antibody antibody detected the POLDIP3 Antibody protein (arrow).)

Related Product Information for anti-POLDIP3 antibody

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq].

Product Categories/Family for anti-POLDIP3 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 522020125
• NCBI GeneID: 84271
• NCBI Accession #: NP_001265586.1
• NCBI GenBank Nucleotide #: NM_001278657.1
• UniProt Accession #: Q9BY77; Q009A7; Q5H972; Q6PGN6; Q7Z6Z0; Q9NSP5; Q9NSP6; A8K6F8; A8K6V9
• Molecular Weight: 46,089 Da
• NCBI Official Full Name: polymerase delta-interacting protein 3 isoform 3
• NCBI Official Synonym Full Names: polymerase (DNA-directed), delta interacting protein 3
• NCBI Official Symbol: POLDIP3
• NCBI Official Synonym Symbols: SKAR; PDIP46
• NCBI Protein Information: polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; 46 kDa DNA polymerase delta interaction protein
• UniProt Protein Name: Polymerase delta-interacting protein 3
• Protein Family: Polymerase delta-interacting protein
• UniProt Gene Name: POLDIP3
• UniProt Synonym Gene Names: KIAA1649; PDIP46; p46; SKAR
• UniProt Entry Name: PDIP3_HUMAN

NCBI Description

This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: protein binding; nucleotide binding

Biological Process: poly(A)+ mRNA export from nucleus; positive regulation of translation