Typical Testing Data/Standard Curve (for reference only)
Canine Adiponectin Receptor 2 ELISA Kit | ADIPOR2 elisa kit
Canine Adiponectin Receptor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ADIPOR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADIPOR2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ADIPOR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADIPOR2 antibody and an ADIPOR2-HRP conjugate. The assay sample and buffer are incubated together with ADIPOR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADIPOR2 concentration since ADIPOR2 from samples and ADIPOR2-HRP conjugate compete for the anti-ADIPOR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADIPOR2 from the sample, fewer sites are left to bind ADIPOR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADIPOR2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008]
Uniprot Description
ADIPOR2: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. Belongs to the ADIPOR family.
Protein type: Membrane protein, multi-pass; Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: integral to membrane; plasma membrane
Molecular Function: adiponectin binding; identical protein binding; hormone binding; protein heterodimerization activity; receptor activity
Biological Process: positive regulation of glucose import; hormone-mediated signaling; heart development; adiponectin-mediated signaling pathway; fatty acid oxidation; negative regulation of cell growth; female pregnancy; response to nutrient
Research Articles on ADIPOR2
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Product Notes
The Canine ADIPOR2 adipor2 (Catalog #AAA739340) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739340 ELISA Kit recognizes Canine ADIPOR2. It is sometimes possible for the material contained within the vial of "Adiponectin Receptor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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