Rabbit POLDIP3 Polyclonal Antibody | anti-POLDIP3 antibody

Anti-POLDIP3 Antibody

Cat. Num. AAA416131

• Gene Names: POLDIP3; SKAR; PDIP46
• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: POLDIP3; Polyclonal Antibody; Anti-POLDIP3 Antibody; anti-POLDIP3 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2042

• Applicable Applications for anti-POLDIP3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal POLDIP3 (1) antibody was raised against a recombinated human POLDIP3 protein 4-149aa (BC019643).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-POLDIP3 antibody

This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

References

(1) Ma,X.M., Yoon,S.O., Richardson,C.J., et al. SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. Cell 133 (2), 303-313 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 18043106
• NCBI GeneID: 84271
• Molecular Weight: 42,895 Da
• NCBI Official Full Name: Homo sapiens polymerase (DNA-directed), delta interacting protein 3, mRNA
• NCBI Official Synonym Full Names: polymerase (DNA-directed), delta interacting protein 3
• NCBI Official Symbol: POLDIP3
• NCBI Official Synonym Symbols: SKAR; PDIP46
• NCBI Protein Information: polymerase delta-interacting protein 3; 46 kDa DNA polymerase delta interaction protein; RNA-binding protein P46; S6K1 Aly/REF-like target
• UniProt Protein Name: Polymerase delta-interacting protein 3
• Protein Family: Polymerase delta-interacting protein
• UniProt Gene Name: POLDIP3
• UniProt Entry Name: PDIP3_HUMAN

NCBI Description

This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: protein binding; nucleotide binding

Biological Process: poly(A)+ mRNA export from nucleus; positive regulation of translation