Rabbit anti-Human POLD1 Polyclonal Antibody | anti-POLD1 antibody

POLD1, ID (POLD1, POLD, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125) (MaxLight 550)

Cat. Num. AAA6335572

• Gene Names: POLD1; CDC2; POLD; CRCS10
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: POLD1; Polyclonal Antibody; ID (POLD1; POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125) (MaxLight 550); DNA polymerase subunit delta p125; anti-POLD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-POLD1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: POLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717~747 amino acids from the Center region of human POLD1.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of POLD1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). POLD1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(POLD1 Antibody (Center) flow cytometry analysis of Jurkatcells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-POLD1 antibody

MaxLight 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor546, 555, DyLight549, Cy3, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Product Categories/Family for anti-POLD1 antibody

Antibodies; Enzymes; Polymerase

References

Chung D.W., Zhang J., Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991) Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992) Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 379030588
• NCBI GeneID: 5424
• NCBI Accession #: NP_001243778.1
• NCBI GenBank Nucleotide #: NM_001256849.1
• UniProt Accession #: P28340; Q8NER3; Q96H98
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: DNA polymerase delta catalytic subunit
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit; CDC2 homolog; DNA polymerase subunit delta p125; polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: EC 2.7.7.7; Transferase; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Hydrolase; Nucleotide Metabolism - purine; DNA replication

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; delta DNA polymerase complex; nucleotide-excision repair complex; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; 3'-5'-exodeoxyribonuclease activity; chromatin binding

Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; DNA replication proofreading; nucleotide-excision repair; base-excision repair; fatty acid homeostasis; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA catabolic process, exonucleolytic; telomere maintenance; response to UV

Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome