Western Blot (WB)
(Anti- POLB Picoband antibody, MBS178313, Western blottingAll lanes: Anti POLB (MBS178313) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugPredicted bind size: 39KDObserved bind size: 39KD )
anti-Human POLB Polyclonal Antibody | anti-POLB antibody
Anti-POLB Antibody
Western Blot (WB)
(Anti- POLB Picoband antibody, MBS178313, Western blottingAll lanes: Anti POLB (MBS178313) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugPredicted bind size: 39KDObserved bind size: 39KD )
Western Blot (WB)
(Anti- POLB Picoband antibody, MBS178313, Western blottingAll lanes: Anti POLB (MBS178313) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugPredicted bind size: 39KDObserved bind size: 39KD )
Background: Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
Uniprot Description
POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.
Protein type: DNA repair, damage; Lyase; DNA replication; Transferase; EC 2.7.7.7
Chromosomal Location of Human Ortholog: 8p11.2
Cellular Component: cytoplasm; microtubule; nucleoplasm; nucleus; protein complex; spindle microtubule
Molecular Function: damaged DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; DNA-directed DNA polymerase activity; enzyme binding; lyase activity; metal ion binding; microtubule binding; protein binding
Biological Process: aging; base-excision repair; base-excision repair, base-free sugar-phosphate removal; base-excision repair, DNA ligation; base-excision repair, gap-filling; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; DNA-dependent DNA replication; homeostasis of number of cells; inflammatory response; lymph node development; neuron apoptosis; nucleotide-excision repair, DNA gap filling; pyrimidine dimer repair; response to DNA damage stimulus; response to ethanol; response to gamma radiation; response to hyperoxia; salivary gland morphogenesis; somatic hypermutation of immunoglobulin genes; spleen development
Research Articles on POLB
Similar Products
Product Notes
The POLB polb (Catalog #AAA178313) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-POLB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the POLB polb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
