Rabbit anti-Human POFUT2 Polyclonal Antibody | anti-POFUT2 antibody

POFUT2, ID (POFUT2, C21orf80, FUT13, KIAA0958, GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2) (FITC)

Cat. Num. AAA6335535

• Gene Names: POFUT2; FUT13; C21orf80
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POFUT2; Polyclonal Antibody; ID (POFUT2; C21orf80; FUT13; KIAA0958; GDP-fucose protein O-fucosyltransferase 2; Peptide-O-fucosyltransferase 2) (FITC); Peptide-O-fucosyltransferase 2; anti-POFUT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 429

• Applicable Applications for anti-POFUT2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-265 amino acids from the Central region of human POFUT2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POFUT2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the POFUT2 antibody detected the POFUT2 protein (arrow).)

Immunohistochemistry (IHC)

(POFUT2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POFUT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-POFUT2 antibody

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).

Product Categories/Family for anti-POFUT2 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Feng, T., et al. Hum. Genet. 128(3):269-280(2010). Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006). Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006). Menzel, O., et al. Genomics 84(2):320-330(2004). Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 34147487
• NCBI GeneID: 23275
• NCBI Accession #: NP_598368.2
• NCBI GenBank Nucleotide #: NP_598368.2
• UniProt Accession #: Q9Y2G5
• NCBI Official Full Name: GDP-fucose protein O-fucosyltransferase 2 isoform C
• NCBI Official Synonym Full Names: protein O-fucosyltransferase 2
• NCBI Official Symbol: POFUT2
• NCBI Official Synonym Symbols: FUT13; C21orf80
• NCBI Protein Information: GDP-fucose protein O-fucosyltransferase 2
• UniProt Protein Name: GDP-fucose protein O-fucosyltransferase 2
• UniProt Gene Name: POFUT2
• UniProt Synonym Gene Names: C21orf80; FUT13; KIAA0958; O-FucT-2
• UniProt Entry Name: OFUT2_HUMAN

NCBI Description

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

Uniprot Description

POFUT2: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.221

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane

Molecular Function: peptide-O-fucosyltransferase activity

Biological Process: fucose metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; mesoderm formation; regulation of gene expression; regulation of secretion; post-translational protein modification