Rabbit POFUT2 Polyclonal Antibody | anti-POFUT2 antibody

POFUT2 Polyclonal Antibody

Cat. Num. AAA3009671

• Gene Names: POFUT2; FUT13; C21orf80
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POFUT2; Polyclonal Antibody; POFUT2 Polyclonal Antibody; FUT13; C21orf80; anti-POFUT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POFUT2 polyclonal antibody detects endogenous levels of POFUT2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 141

• Applicable Applications for anti-POFUT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human POFUT2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using POFUT2 antibody.)

Related Product Information for anti-POFUT2 antibody

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).

NCBI and Uniprot Product Information

• NCBI GI #: 45708525
• NCBI GeneID: 23275
• UniProt Accession #: Q9Y2G5
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: POFUT2 protein
• NCBI Official Synonym Full Names: protein O-fucosyltransferase 2
• NCBI Official Symbol: POFUT2
• NCBI Official Synonym Symbols: FUT13; C21orf80
• NCBI Protein Information: GDP-fucose protein O-fucosyltransferase 2
• UniProt Protein Name: GDP-fucose protein O-fucosyltransferase 2
• UniProt Gene Name: POFUT2
• UniProt Synonym Gene Names: C21orf80; FUT13; KIAA0958; O-FucT-2
• UniProt Entry Name: OFUT2_HUMAN

NCBI Description

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

Uniprot Description

POFUT2: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.221

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane

Molecular Function: peptide-O-fucosyltransferase activity

Biological Process: fucose metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; mesoderm formation; regulation of gene expression; regulation of secretion; post-translational protein modification