Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Podocin (PDCN) Polyclonal Antibody | anti-PDCN antibody
Polyclonal Antibody to Podocin (PDCN)
Gene Names
Nphs2; PDCN; SRN1
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Podocin (PDCN); Polyclonal Antibody; Polyclonal Antibody to Podocin (PDCN); anti-PDCN antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PDCN. It has been selected for its ability to recognize PDCN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-IFR LGHLLPGRAK GPGLFFFLPC LDTYHKVDLR LQTLEIPFHE VVTKDMFIME IDAVCYYRME NASLLLSSLA HVSKAIQFLV QTTMKRLLAH RSLTEILLER KSIAQDVKVA LDAVTCIWGI KVERTEIKDV RLPAGLQHSL AVEAEAQRQA KVRVIAAEGE KAASESLRMA AEILSGTPAA VQLRYLHTLQ SLSTEKPATV VLPLPFDMLS LLSSPGNRAQ GSINYPSSSK P
MGHHHHHHSGSEF-IFR LGHLLPGRAK GPGLFFFLPC LDTYHKVDLR LQTLEIPFHE VVTKDMFIME IDAVCYYRME NASLLLSSLA HVSKAIQFLV QTTMKRLLAH RSLTEILLER KSIAQDVKVA LDAVTCIWGI KVERTEIKDV RLPAGLQHSL AVEAEAQRQA KVRVIAAEGE KAASESLRMA AEILSGTPAA VQLRYLHTLQ SLSTEKPATV VLPLPFDMLS LLSSPGNRAQ GSINYPSSSK P
Sequence Length
383
Applicable Applications for anti-PDCN antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PDCN (Ile138~Pro371) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049727
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,337 Da
NCBI Official Full Name
podocin
NCBI Official Synonym Full Names
nephrosis 2, podocin
NCBI Official Symbol
Nphs2
NCBI Official Synonym Symbols
PDCN; SRN1
NCBI Protein Information
podocin
UniProt Protein Name
Podocin
UniProt Gene Name
Nphs2
Uniprot Description
NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1|1 G3
Cellular Component: integral component of membrane; integral component of plasma membrane; intercellular junction; membrane; membrane raft; plasma membrane; protein complex
Molecular Function: protein binding
Biological Process: actin cytoskeleton reorganization; excretion
Research Articles on PDCN
Similar Products
Product Notes
The PDCN nphs2 (Catalog #AAA2005156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Podocin (PDCN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Podocin (PDCN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PDCN nphs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-IFR LGHLLPGRAK GPGLFFFLPC LDTYHKVDLR LQTLEIPFHE VVTKDMFIME IDAVCYYRME NASLLLSSLA HVSKAIQFLV QTTMKRLLAH RSLTEILLER KSIAQDVKVA LDAVTCIWGI KVERTEIKDV RLPAGLQHSL AVEAEAQRQA KVRVIAAEGE KAASESLRMA AEILSGTPAA VQLRYLHTLQ SLSTEKPATV VLPLPFDMLS LLSSPGNRAQ GSINYPSSSK P. It is sometimes possible for the material contained within the vial of "Podocin (PDCN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
