Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNLDC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 15s.)
Rabbit PNLDC1 Polyclonal Antibody | anti-PNLDC1 antibody
PNLDC1 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
PNLDC1; Polyclonal Antibody; PNLDC1 Rabbit pAb; HsPNLDC1; anti-PNLDC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
DEGVVVKKVSKQHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLHEKFFRPLPESYDQFKQNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIVHASRCEKYVETKCPHEAAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRAGVPKINFSGPDYPSIRPPILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNILKEYRDHPTLCISLYRYWRHSPNVNCLLQ
Applicable Applications for anti-PNLDC1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human PNLDC1 (NP_775787.1).
Positive Samples
Raji, U-251MG, Mouse liver, Mouse testis, Mouse brain, Rat testis
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNLDC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 15s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNLDC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 15s.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,124 Da
NCBI Official Full Name
poly(A)-specific ribonuclease PARN-like domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
poly(A)-specific ribonuclease (PARN)-like domain containing 1
NCBI Official Symbol
PNLDC1
NCBI Protein Information
poly(A)-specific ribonuclease PARN-like domain-containing protein 1; poly(A)-specific ribonuclease PARN-like domain-containing protein 1
UniProt Protein Name
Poly(A)-specific ribonuclease PARN-like domain-containing protein 1
UniProt Gene Name
PNLDC1
UniProt Entry Name
PNDC1_HUMAN
Uniprot Description
PNLDC1: Belongs to the CAF1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q25.3
Cellular Component: integral to membrane; nucleus
Molecular Function: nucleic acid binding
Research Articles on PNLDC1
Similar Products
Product Notes
The PNLDC1 pnldc1 (Catalog #AAA9143057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNLDC1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PNLDC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PNLDC1 pnldc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DEGVVVKKVS KQHRWYLQNT SCDRESCWKE NILLSARGFS VFFQMLVKAQ KPLVGHNMMM DLLHLHEKFF RPLPESYDQF KQNIHSLFPV LIDTKSVTKD IWKEMNFPRV SNLSEVYEVL NSDLNPTKNS GPEIVHASRC EKYVETKCPH EAAYDAFLCG SVLLKVAHLL LQKIYHIDPV PESSFPQYLD VLAPYVNQVN LIRAGVPKIN FSGPDYPSIR PPILILSVKR WPGVSEQQVY HKFQNLCKFD VRRLTRSQFL LLTNKFKDAR NILKEYRDHP TLCISLYRYW RHSPNVNCLL Q. It is sometimes possible for the material contained within the vial of "PNLDC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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